GM24076
LCL from B-Lymphocyte
Description:
PERSONAL GENOME PROJECT
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently Healthy Collection
PIGI Consented Sample |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Race
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White
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Country of Origin
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USA
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Family History
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Y
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Relation to Proband
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proband
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by LINE assay |
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| Remarks |
Participant (hu52B7E5) in the Personal Genome Project: http://www.personalgenomes.org
Subject diagnosed with invasive lobular breast cancer at age 39 years; negative for BRCA 1 and 2 mutations as well as CDH1 mutation;other diagnoses include myopia, dry eye syndrome, hemorrhoids, dental cavities, fibrocystic breast disease, skin tags, osteoarthritis, sciatica, and scoliosis; allergies include: hay fever, honey bee venom protein, house dust, and mold; supplements/medications include: calcium, fish oil, folic acid, ginsana, glucosamine-chond-cal-115HC, green tea extract, melatonin, multiple vitamin, tamoxifen, vitamin B-100 complex, and vitamin D-3; previous procedures include: knee arthroscopy, removal of ganglion cyst in the wrist, umbilical hernia repair, breast reconstruction with free skin and muscle flap, sentinel lymph node dissection, mastectomy, axillary lymph node dissection, radiation therapy, and oophorectomy; family history:mother diagnosed with breast cancer at age 43, aunt diagnosed with breast cancer at age 48, and maternal great-grandmother diagnosed with breast cancer at age 43. |
| Smullen M, Olson MN, Reichert JM, Dawes P, Murray LF, Baer CE, Wang Q, Readhead B, Church GM, Lim ET, Chan Y, Reliable multiplex generation of pooled induced pluripotent stem cells Cell reports methods3:100570 2022 |
| PubMed ID: 37751688 |
| |
| Mao Q, Ciotlos S, Zhang RY, Ball MP, Chin R, Carnevali P, Barua N, Nguyen S, Agarwal MR, Clegg T, Connelly A, Vandewege W, Zaranek AW, Estep PW, Church GM, Drmanac R, Peters BA., The whole genome sequences and experimentally phased haplotypes of over 100 personal genomes. Gigascience.5(1):42 2016 |
| PubMed ID: 27724973 |
| |
| Church GM, The personal genome project. Mol Syst Biol.1, 2005.0030:42 2005 |
| PubMed ID: 16729065 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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