GM20431
LCL from B-Lymphocyte
Description:
PERSONAL GENOME PROJECT
APPARENTLY HEALTHY INDIVIDUAL
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Apparently Healthy Collection
PIGI Consented Sample
Whole-Genome Sequence |
|
Biopsy Source
|
Peripheral vein
|
|
Cell Type
|
B-Lymphocyte
|
|
Tissue Type
|
Blood
|
|
Transformant
|
Epstein-Barr Virus
|
|
Sample Source
|
LCL from B-Lymphocyte
|
|
Race
|
White
|
|
Country of Origin
|
USA
|
|
Family Member
|
1
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
|
ISCN
|
arr[hg19] 9p24.2p23(3,957,865-10,280,656)x1
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin confirmed by LINE assay |
| |
| Demographic Data |
| Relation to Proband |
proband |
| Age at Sampling |
51 YR |
| Sex |
Male |
| Racial Category |
White |
| Country |
USA |
| |
| Data Elements |
| Clinical Element Type: Church PHGP |
| (Baseline) |
| Vital Signs |
| Blood Type |
O+ |
| Height |
6'4" |
| Weight |
245 |
| Blood Pressure - Systolic (mm Hg) |
120 |
| Blood Pressure - Diastolic (mm Hg) |
80 |
| Allergies |
| Substance |
NONE |
| Medications, Vitamins, Supplements |
| Medication |
LOVASTATIN, COQ (100MG) CALCIUM + VIT D, VIT C, OMEGA FATTY ACIDS |
| Medical History |
| Medical Condition |
NARCOLEPSY, HYPERLIPIDEMIA, IRITIS, HYPERSOMNOLENCE, SQUAMOUS CELL CARCINOMA, CHEST PAIN |
| Race, Ethnicity, Ancestry |
| Race |
WHITE |
| Country of Origin - Subject |
USA |
| Country of Origin - Paternal Grandfather |
USA |
| Country of Origin - Paternal Grandmother |
USA |
| Country of Origin - Maternal Grandfather |
USA |
| Country of Origin - Maternal Grandmother |
USA |
| Traits |
| Eye Color |
green |
| Dominant Hand |
right |
| Near-sighted |
Yes No |
| Far-sighted |
Yes No |
| Wear Prescription Glasses |
Yes No |
| Remarks |
Participant #1 (hu43860C) in the Personal Genome Project: http://www.personalgenomes.org
Matching fibroblast culture from this same subject is GM23248. Whole genome sequence data is available on the PGP website. |
| Smullen M, Olson MN, Reichert JM, Dawes P, Murray LF, Baer CE, Wang Q, Readhead B, Church GM, Lim ET, Chan Y, Reliable multiplex generation of pooled induced pluripotent stem cells Cell reports methods3:100570 2022 |
| PubMed ID: 37751688 |
| |
| Caballero M, Ge T, Rebelo AR, Seo S, Kim S, Brooks K, Zuccaro M, Kanagaraj R, Vershkov D, Kim D, Smogorzewska A, Smolka M, Benvenisty N, West SC, Egli D, Mace EM, Koren A, Comprehensive analysis of DNA replication timing across 184 cell lines suggests a role for MCM10 in replication timing regulation Human molecular genetics3:100570 2021 |
| PubMed ID: 35394024 |
| |
| Mao Q, Ciotlos S, Zhang RY, Ball MP, Chin R, Carnevali P, Barua N, Nguyen S, Agarwal MR, Clegg T, Connelly A, Vandewege W, Zaranek AW, Estep PW, Church GM, Drmanac R, Peters BA., The whole genome sequences and experimentally phased haplotypes of over 100 personal genomes. Gigascience.5(1):42 2016 |
| PubMed ID: 27724973 |
| |
| Peters BA1, Kermani BG, Sparks AB, Alferov O, Hong P, Alexeev A, Jiang Y, Dahl F, Tang YT, Haas J, Robasky K, Zaranek AW, Lee JH, Ball MP, Peterson JE, Perazich H, Yeung G, Liu J, Chen L, Kennemer MI, Pothuraju K, Konvicka K, Tsoupko-Sitnikov M, Pant KP, Ebert JC, Nilsen GB, Baccash J, Halpern AL, Church GM, Drmanac R., Accurate whole-genome sequencing and haplotyping from 10 to 20 human cells. Nature487(7406):190-5 2012 |
| PubMed ID: 22785314 |
| |
| Drmanac R1, Sparks AB, Callow MJ, Halpern AL, Burns NL, Kermani BG, Carnevali P, Nazarenko I, Nilsen GB, Yeung G, Dahl F, Fernandez A, Staker B, Pant KP, Baccash J, Borcherding AP, Brownley A, Cedeno R, Chen L, Chernikoff D, Cheung A, Chirita R, Curson B, Ebert JC, Hacker CR, Hartlage R, Hauser B, Huang S, Jiang Y, Karpinchyk V, Koenig M, Kong C, Landers T, Le C, Liu J, McBride CE, Morenzoni M, Morey RE, Mutch K, Perazich H, Perry K, Peters BA, Peterson J, Pethiyagoda CL, Pothuraju K, Richter C, Rosenbaum AM, Roy S, Shafto J, Sharanhovich U, Shannon KW, Sheppy CG, Sun M, Thakuria JV, Tran A, Vu D, Zaranek AW, Wu X, Drmanac S, Oliphant AR, Banyai WC, Martin B, Ballinger DG, Church GM, Reid CA., Human genome sequencing using unchained base reads on self-assembling DNA nanoarrays. Science327(5961):78-81 2010 |
| PubMed ID: 19892942 |
| |
| Church GM, The personal genome project. Mol Syst Biol.1, 2005.0030:78-81 2005 |
| PubMed ID: 16729065 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
|
|