GM15511
Somatic cell hybrid
Description:
CHROMOSOME 5 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
ADDITIONAL DELETION/DERIVATIVE Y CHROMOSOME SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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Human/hamster somatic cell hybrid retaining a psu dic(Y;5)(Ypter>Yq12:: 5p15.1>5qter) (100%) & #3, 13, and 21
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Remarks |
Line JH243; human lymphoblasts, GM14129, 45,X,dic(Y;5)(Ypter>Yq12::5p15.1>5qter), fused with Chin ham UCW56 cells; grown in medium containing 0.2mM proline at 39C; retains hum psu dic (Y;5)(Ypter> Yq12::5p15.1>5qter) in 100% of cells |
| Split Ratio |
1:5 |
| Temperature |
39 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Proline 0.2 mM |
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