GM15292
Somatic cell hybrid
Description:
CHROMOSOME 7 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 10 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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Human/mouse somatic cell hybrid retaining a human der(10)t(7;10)(p22.1;q11.21) in 84% of cells and human #3, 4, 8, der(12)?, 14, 16, 17, 18, 20, 21, 22, & X
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Remarks
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| Remarks |
Line GM0044RAG1-15; produced by the fusion of human line GM00044, 46,XY,del(7)(p21.2p22.1),t(7;10)(7qter>7p21.2::10q11.21>10qter;10pter>10q11.21::7p22.1>7pter), with RAG mouse cells; culture grown without selection; retains human der(10)t(7;10)(p22.1;q11.21) in 84% of cells and other human chromosomes |
| Potapova TA, Unruh JR, Yu Z, Rancati G, Li H, Stampfer MR, Gerton JL, Superresolution microscopy reveals linkages between ribosomal DNA on heterologous chromosomes The Journal of cell biology: 2018 |
| PubMed ID: 31270138 |
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| Vortkamp A, Thias U, Gessler M, Rosenkranz W, Kroisel PM, Tommerup N, Kruger G, Gotz J, Pelz L, Grzeschik KH, A somatic cell hybrid panel and DNA probes for physical mapping of human chromosome 7p. Genomics11:737-43 1991 |
| PubMed ID: 1663489 |
| Split Ratio |
1:8 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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