GM14566
Somatic cell hybrid
Description:
CHROMOSOME X REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 18 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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Human/Chinese hamster hybrid retaining a human der(X)t(X;18)(p11.2;q11.2) in 76% of cells, 4, 7, 12, and 20
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for Xp22.32, STS, and gave a positive result with a primer for Xq21.3, DXS3. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2 |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 2p13, TGFA. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 3q13.1-q13.2, D3S1310, 3q26.2-q27, GLUT2, and 3q28-q29, D3S1311. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5 |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 5p14-p12, C9, and 5q34-q35, GABRA1. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 7 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 7pter-p22, D7S472, 7p12, EGFR, and 7q36-qter, D7S550. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 15 |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 15q21-q22.2, B2M. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 18 |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 18p11.1-q11.2, D18S44, and 18p11.31-p11.22, TYMS, and gave positive results with primers for 18q12.1, TTR, and 18q23, D18S1390. |
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| Remarks |
Line HySynSarc; produced by the fusion of a human synovial sarcoma, t(X;18) (p11.2;q11.2), with HGPRT deficient Chinese hamster CH3S cells; 76% of cells retain a human der(X)t(X;18)(p11.2;q11.2), and also a #4(80%), #7(4%), #12(100%) and #20(44%) |
| Gilgenkrantz S, Chery M, Teboul M, Mujica P, Leotard B, Gregoire MJ, Boman F, Duprez A, Hanauer A, Sublocalisation of the X breakpoint in the translocation (X; 18)(p11.2; q11.2) primary change in synovial sarcomas. Oncogene5:1063-6 1990 |
| PubMed ID: 2165233 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
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