GM14563
Somatic cell hybrid
Description:
CHROMOSOME 18 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Human/Chinese hamster hybrid retaining a human del(18)(q21.33) in 100% of cells
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Line JH306; produced by the fusion of human lymphoblasts, GM50122 (JL184) with karyotype del(18)(pter> q21.33:), with Chinese hamster UCW206 cells; grown at 39C; 100% of cells retain a human del(18)(q21.33) |
| Katz SG, Schneider SS, Bartuski A, Trask BJ, Massa H, Overhauser J, Lalande M, Lansdorp PM, Silverman GA, An 18q- syndrome breakpoint resides between the duplicated serpins SCCA1 and SCCA2 and arises via a cryptic rearrangement with satellite III DNA. Hum Mol Genet8(1):87-92 1999 |
| PubMed ID: 9887335 |
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| Strathdee G, Zackai EH, Shapiro R, Kamholz J, Overhauser J, Analysis of clinical variation seen in patients with 18q terminal deletions. Am J Med Genet59(4):476-83 1995 |
| PubMed ID: 8585568 |
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| Kline AD, White ME, Wapner R, Rojas K, Biesecker LG, Kamholz J, Zackai EH, Muenke M, Scott CI Jr, Overhauser J, Molecular analysis of the 18q- syndrome--and correlation with phenotype. Am J Hum Genet52(5):895-906 1993 |
| PubMed ID: 8488839 |
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| Rojas K, Overhauser J, Sublocalization of 21 chromosome 18-specific microsatellite markers. Genomics18:169-71 1993 |
| PubMed ID: 8276412 |
| Split Ratio |
1:20 |
| Temperature |
39 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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