GM14416
Somatic cell hybrid
Description:
CHROMOSOME 21 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 6 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and Molecular characterization
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ISCN
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Human/hamster hybrid retaining a der(6) t(6;21)(p25;q11) in 96% of cells, add(3) (p?) (96%) , #12 (96%), & X (92%)
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 6 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 6q24-q27, MAS1, 6q21-q23.3, D6S292, 6q27-qter, D6S297, and 6p25, D6S344. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 21 |
PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 21q21.2, AMYLOID, and gave negative results with primers for 21p11.2, D21S1278, 21q11, D21S120, and 21q11.1, D21S236. |
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| Remarks |
Line R50-3; produced by the fusion of GM00144, 45,XX,-6,-21,+der(6)t(6;21), with pyrimidine auxotrophic CHO line, J405; selected and grown in purine-free medium; retains hum der(6)t(6;21) (96%), add(3)(p?) (96%), #12 (96%), & X (92%) |
| Chastain-Moore AM, Roberts T, Trott DA, Newbold RF, Ornelles DA, An activity associated with human chromosome 21 permits nuclear colocalization of the adenovirus E1B-55K and E4orf6 proteins and promotes viral late gene expression. J Virol77(14):8087-98 2003 |
| PubMed ID: 12829847 |
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| Graw SL, Gardiner K, Hall-Johnson K, Hart I, Joetham A, Walton K, Donaldson D, Patterson D, Molecular analysis and breakpoint definition of a set of human chromosome 21 somatic cell hybrids. Somat Cell Mol Genet21:415-428 1995 |
| PubMed ID: 8600569 |
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| Gardiner K, Watkins P, Munke M, Drabkin H, Jones C, Patterson D, Partial physical map of human chromosome 21. Somat Cell Mol Genet14:623-37 1988 |
| PubMed ID: 3194801 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Ham's F12 deficient Medium with 2mM L-glutamine or equivalent |
| Serum |
5% dialyzed fetal bovine serum |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Proline 50mg/L |
| Supplement |
Uridine 3 x 10-5M |
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