GM13446
Somatic cell hybrid
Description:
CHROMOSOME 6 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and In situ hybridization
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ISCN
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Chinese hamster/human hybrid retaining human 6cen>6qter, #9, #10, & Y
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - Y CHROMOSOME |
PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for Yq11, DYS227. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 6 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 6q24-q27, MAS1, 6q21-q23.3, D6S292, and 6q27qter, D6S297, and gave negative results with primers for 6p21.3, TNFA, and 6p25, D6S344. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 10 |
PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 10q24, PLAU. |
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| Remarks |
Line 640-5A; human/Chinese hamster ovary somatic cell hybrid retaining human 6cen>6qter translocated to a rodent chromosome in 100% of cells & #9, #10, & Y; grown in culture medium without selection |
| Pappas GJ, Polymeropoulos MH, Boyle JM, Trent JM, Regional assignment by hybrid mapping of 36 expressed sequence tags (ESTs) on human chromosome 6. Genomics25:124-9 1995 |
| PubMed ID: 7774909 |
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| Meese EU, Meltzer PS, Ferguson PW, Trent JM, Alu-PCR: characterization of a chromosome 6-specific hybrid mapping panel and cloning of chromosome-specific markers. Genomics12:549-54 1992 |
| PubMed ID: 1559706 |
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| Meese EU, Witkowski CM, Zoghbi HY, Stanbridge EJ, Meltzer PS, Trent JM, Development and utilization of a somatic cell hybrid mapping panel to assign NotI linking probes to the long arm of human chromosome 6. Genomics12:542-8 1992 |
| PubMed ID: 1559705 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F12 with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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