GM13139
Somatic cell hybrid
Description:
NIGMS MAPPING PANEL #2, VERSION 3, HUMAN/RODENT SOMATIC CELL HYBRIDS
NIGMS HUMAN/RODENT SOMATIC CELL HYBRID MAPPING PANEL #2 DNA
NIGMS HUMAN/RODENT SOMATIC CELL HYBRID MINI MAPPING PANEL #2 DNA
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and In situ hybridization
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ISCN
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Human/mouse somatic cell hybrid retaining human chromosome #1
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for Xq28, DXS15. S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for Xp22.32, STS, Xq13.3, PGK1, and Xq28, G6PD. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 1 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 1p36, PND. S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 1p13, NGFB, and 1q32, REN. PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 1p13, NGFB. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2 |
S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 2p13, TGFA, 2p23, POMC, and 2q13, IL1A. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 |
S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 3p, MYL3, 3p22-p21.33, GLB1, 3q21.3-q25, ACPP, and 3q26.2-q27, GLUT2. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 4 |
S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 4p15.3, QDPR, and 4q11-q13, AFP. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 5pterp15.3, D5S10. S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 5p14-p12, C9, and 5q13.3-q14, HMGCR. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 6 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 6q24q27, ESR. S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 6p21.3, TNFA, and 6q24-q27, MAS1. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 7 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 7pterp15, ASSP11. S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 7p12, EGFR, and 7q31.3, CFTR. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 8 |
S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 8p21-p11.2, LHRH, 8p23-p22, DEF1, and 8q24, TG. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 9 |
S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 9p22, IFNA, and 9q22.3-q31, ALDOB. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 10 |
S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 10p11.2, ITGB1, and 10q24, PLAU. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 11 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 11q23.3, ETS1. S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 11p13, CAT, and 11q21-q22, CLG. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 12 |
S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 12p12.1, KRAS, 12p13.3-p13.2, F8VWF, and 12q22-q24.2, PAH. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 13 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 13q34, D13S3. S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 13q14.3, RB1. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 14 |
S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 14q11.2, NP, and 14q32.1, PI. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 15 |
S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 15q21-q22.2, B2M. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 16 |
S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 16p13.3, HBA, and 16q22.1, LCAT. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 |
S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 17p13.1, TP53, 17q, GAS, and 17q21.3-q23, MPO. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 18 |
S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 18p11.31-p11.22, TYMS. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 19 |
S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 19p13.2, LDLR, and 19q13.2, APOC2. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 20 |
S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 20pter-p12, PRNP, and 20q12q13.11, ADA. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 21 |
S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 21q22.3, ITGB2. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22 |
S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 22q11.2, IGLC2. |
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| Remarks |
Line A9-1neo; produced by the fusion of pSV2 neo transfected MRC5 fibro with mouse A9 cells; selected in medium containing G-418; retains human chromosome #1 in 100% of cells |
| Pironon N, Puechberty J, Roizès G, Molecular and evolutionary characteristics of the fraction of human alpha satellite DNA associated with CENP-A at the centromeres of chromosomes 1, 5, 19, and 21 BMC genomics11:195 2009 |
| PubMed ID: 20331851 |
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| Krona C, Ejeskär K, Carén H, Abel F, Sjöberg RM, Martinsson T, A novel 1p362 located gene, APITD1, with tumour-suppressive properties and a putative p53-binding domain, shows low expression in neuroblastoma tumours British journal of cancer91:1119-30 2004 |
| PubMed ID: 15328517 |
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| Mallet F, Bouton O, Prudhomme S, Cheynet V, Oriol G, Bonnaud B, Lucotte G, Duret L, Mandrand B, The endogenous retroviral locus ERVWE1 is a bona fide gene involved in hominoid placental physiology Proceedings of the National Academy of Sciences of the United States of America101:1731-6 2004 |
| PubMed ID: 14757826 |
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| Conte C, Ebeling M, Marcuz A, Nef P, Andres-Barquin PJ, Identification and characterization of human taste receptor genes belonging to the TAS2R family Cytogenetic and genome research98:45-53 2003 |
| PubMed ID: 12584440 |
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| Hansford LM, Smith SA, Haber M, Norris MD, Cheung B, Marshall GM, Cloning and characterization of the human neural cell adhesion molecule, CNTN4 (alias BIG-2) Cytogenetic and genome research101:17-23 2003 |
| PubMed ID: 14571131 |
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| Janssens B, Mohapatra B, Vatta M, Goossens S, Vanpoucke G, Kools P, Montoye T, van Hengel J, Bowles NE, van Roy F, Towbin JA, Assessment of the CTNNA3 gene encoding human alpha T-catenin regarding its involvement in dilated cardiomyopathy Human genetics112:227-36 2003 |
| PubMed ID: 12596047 |
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| Tarafa G, Prat E, Risques RA, González S, Camps J, Grau M, Guinó E, Moreno V, Esteller M, Herman JG, Germà JR, Miró R, Peinado MA, Capellá G, Common genetic evolutionary pathways in familial adenomatous polyposis tumors Cancer research63:5731-7 2003 |
| PubMed ID: 14522893 |
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| Thameem F, Yang X, Permana PA, Wolford JK, Bogardus C, Prochazka M, Evaluation of the microsomal glutathione S-transferase 3 (MGST3) locus on 1q23 as a Type 2 diabetes susceptibility gene in Pima Indians Human genetics113:353-8 2003 |
| PubMed ID: 12898215 |
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| Wolford JK, Gruber JD, Ossowski VM, Vozarova B, Antonio Tataranni P, Bogardus C, Hanson RL, A C-reactive protein promoter polymorphism is associated with type 2 diabetes mellitus in Pima Indians Molecular genetics and metabolism78:136-44 2003 |
| PubMed ID: 12618085 |
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| Vollrath D, Jaramillo-Babb VL, A sequence-ready BAC clone contig of a 2.2-Mb segment of human chromosome 1q24. Genome Res9:150-7 1999 |
| PubMed ID: 10022979 |
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| Besirli CG, Gong TW, Lomax MI, The Atp1b3 gene for Na,K-ATPase beta 3 subunit maps to mouse chromosome 9, and a related gene, Atp1b3-rs, maps to mouse chromosome 3. Mamm Genome9:171-2 1998 |
| PubMed ID: 9457684 |
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| Tiranti V, Hoertnagel K, Carrozzo R, Galimberti C, Munaro M, Granatiero M, Zelante L, Gasparini P, Marzella R, Rocchi M, Bayona-Bafaluy MP, Enriquez JA, Uziel G, Bertini E, Dionisi-Vici C, Franco B, Meitinger T, Zeviani M, Mutations of SURF-1 in leigh disease associated with cytochrome c oxidase deficiency [In Process Citation] Am J Hum Genet63:1609-21 1998 |
| PubMed ID: 9837813 |
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| Iannotti CA, Inoue H, Bernal E, Aoki M, Liu L, Donis-Keller H, German MS, Permutt MA, Identification of a human LMX1 (LMX1.1)-related gene, LMX1.2: tissue- specific expression and linkage mapping on chromosome 9. Genomics46:520-4 1997 |
| PubMed ID: 9441763 |
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| Jones MH, Zhang Y, Tirosvoutis KN, Davey PM, Webster AR, Walsh D, Spurr NK, Affara NA, Chromosomal assignment of 311 sequences transcribed in human adult testis. Genomics40:155-67 1997 |
| PubMed ID: 9070934 |
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| Schwarz H, Arden K, Lotz M, CD137, a member of the tumor necrosis factor receptor family, is located on chromosome 1p36, in a cluster of related genes, and colocalizes with several malignancies Biochemical and biophysical research communications235:699-703 1997 |
| PubMed ID: 9207223 |
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| Thompson DB, Sutherland J, Apel W, Ossowski V, A physical map at 1p31 encompassing the acute insulin response locus and the leptin receptor. Genomics39:227-30 1997 |
| PubMed ID: 9027510 |
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| White RA, Adkison LR, Dowler LL, Ray RB, Chromosomal localization of the human gene encoding c-myc promoter- binding protein (MPB1) to chromosome 1p35-pter. Genomics39:406-8 1997 |
| PubMed ID: 9119380 |
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| Finelli P, Antonacci R, Marzella R, Lonoce A, Archidiacono N, Rocchi M, Structural organization of multiple alphoid subsets coexisting on human chromosomes 1, 4, 5, 7, 9, 15, 18, and 19. Genomics38:325-30 1996 |
| PubMed ID: 8975709 |
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| Hirai M, Kusuda J, Hashimoto K, Assignment of human ADP ribosylation factor (ARF) genes ARF1 and ARF3 to chromosomes 1q42 and 12q13, respectively. Genomics34(2):263-5 1996 |
| PubMed ID: 8661066 |
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| van Bokhoven H, Rawson RB, Merkx GF, Cremers FP, Seabra MC, cDNA cloning and chromosomal localization of the genes encoding the alpha- and beta-subunits of human Rab geranylgeranyl transferase: the 3' end of the alpha-subunit gene overlaps with the transglutaminase 1 gene promoter. Genomics38:133-40 1996 |
| PubMed ID: 8954794 |
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| Weterman MA, Wilbrink M, Dijkhuizen T, van den Berg E, Geurts van Kessel A, Fine mapping of the 1q21 breakpoint of the papillary renal cell carcinoma-associated (X;1) translocation. Hum Genet98:16-21 1996 |
| PubMed ID: 8682500 |
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| Weterman MA, Wilbrink M, Geurts van Kessel A, Fusion of the transcription factor TFE3 gene to a novel gene, PRCC, in t(X;1)(p11;q21)-positive papillary renal cell carcinomas. Proc Natl Acad Sci U S A93:15294-8 1996 |
| PubMed ID: 8986805 |
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| Whitney M, Thayer M, Reifsteck C, Olson S, Smith L, Jakobs PM, Leach R, Naylor S, Joenje H, Grompe M, Microcell mediated chromosome transfer maps the Fanconi anaemia group D gene to chromosome 3p. Nat Genet11:341-3 1995 |
| PubMed ID: 7581463 |
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| Kugoh HM, Hashiba H, Shimizu M, Oshimura M, Suggestive evidence for functionally distinct, tumor-suppressor genes on chromosomes 1 and 11 for a human fibrosarcoma cell line, HT1080. Oncogene5:1637-44 1990 |
| PubMed ID: 2267132 |
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| Sugawara O, Oshimura M, Koi M, Annab LA, Barrett JC, Induction of cellular senescence in immortalized cells by human chromosome 1. Science247:707-10 1990 |
| PubMed ID: 2300822 |
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| Yamada H, Wake N, Fujimoto S, Barrett JC, Oshimura M, Multiple chromosomes carrying tumor suppressor activity for a uterine endometrial carcinoma cell line identified by microcell-mediated chromosome transfer. Oncogene5:1141-7 1990 |
| PubMed ID: 2392323 |
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| Koi M, Shimizu M, Morita H, Yamada H, Oshimura M, Construction of mouse A9 clones containing a single human chromosome tagged with neomycin-resistance gene via microcell fusion. Jpn J Cancer Res80:413-8 1989 |
| PubMed ID: 2502516 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Geneticin 250ug/ml |
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