GM13080
Somatic cell hybrid
Description:
CHROMOSOME 18 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and In situ hybridization
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ISCN
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Human/Chinese hamster hybrid retaining a human der(18)t(4;18) & #3
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 4 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 4p16.2p15.1, D4S20, and 4q21q23, ADH3. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 4p15.3, QDPR, 4q11-q13, AFP, and 4q31-q33, D4S1626. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 18 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 18q23, MBP, and gave a positive result with a probe for 18q12.3, D18S6. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 18p11.31-p11.22, TYMS, 18q12.1, TTR, 18p11.1q11.2, D18S44, and a different primer for the same locus, and gave a negative result with a primer for 18q23-qter, D18S844. |
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| Remarks |
Line JH226; produced by the fusion of human lymphoblasts, 46,XX,t(4;18)(q35; q22.2), with Chinese hamster UCW206 cells; grown in medium with 0.2mM proline at 39C; retains hum der(18)t(4;18) in 100% of cells & #3 in 88% of cells |
| van Kessel AG, Straub RE, Silverman GA, Gerken S, Overhauser J, Report and abstracts of the second international workshop on human chromosome 18 mapping. Doorwerth, the Netherlands, July 19-20, 1993. Cytogenet Cell Genet65:142-65 1994 |
| PubMed ID: 8222751 |
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| Kline AD, Rojas K, Mewar R, Moshinsky D, Overhauser J, Somatic cell hybrid deletion map of human chromosome 18. Genomics13:1-6 1992 |
| PubMed ID: 1577474 |
| Split Ratio |
1:5 |
| Temperature |
39 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Proline 0.2 mM |
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