GM12085
Somatic cell hybrid
Description:
CHROMOSOME 18 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Chinese hamster/human hybrid retaining a der(18) & human #1, 3, 4, 6, 7, 8, 10, 12, 16, 19, 20, 22, & Y
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 9 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 9p22, IFNB, and 9q34.1, ABL. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 13 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 13q34, D13S3. PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 13q11-q12, D13S232, and gave positive results with primers for 13q14.3, RB1, and 13q22-q31, D13S317. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 15 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 15q25qter, IGF1R. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 18 |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 18q12.3, D18S6, and 18q23, MBP. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 18p11.31-p11.22, TYMS, 18q12.1, TTR, 18p11.1q11.2, D18S44, a different primer for the same locus, and 18q23-qter, D18S844. |
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| Remarks |
Line A5832-14-11; human fibro, GM05832, 46,XY,-18,+der(18)t(13;18)(q14.1;q23), fused with TK-def Chinese hamster a23 cells; medium contains hypoxanthine, methotrexate, & thymidine; 96% of cells have hum 18pter>18q23 & other hum chromo |
| van Kessel AG, Straub RE, Silverman GA, Gerken S, Overhauser J, Report and abstracts of the second international workshop on human chromosome 18 mapping. Doorwerth, the Netherlands, July 19-20, 1993. Cytogenet Cell Genet65:142-65 1994 |
| PubMed ID: 8222751 |
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| Markie D, Jones TA, Sheer D, Bodmer WF, A somatic cell hybrid panel for regional mapping of human chromosome 18. Genomics14:431-6 1992 |
| PubMed ID: 1427858 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
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