GM11979
Somatic cell hybrid
Description:
CHROMOSOME 21 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Chinese hamster/human hybrid retaining der(4)t(4;21)(p16.3;q11.2), #3, 6, 8, 16, & 22
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 4 |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 4p16.2p15.1, D4S20, and 4q21q23, ADH3. PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 4p16.3, D4S126, and gave positive results with primers for 4p15.3, QDPR, 4q11-q13, AFP, 4q25, EGF, 4q28, FGA, 4q31q33, D4S1626, and 4q35, F11. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 21 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 21q22.1q22.2, D21S17. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 21q21.2, APP, and 21q22.3, ITGB2, and gave negative results with primers for 21q11, D21S120, and 21q11.1, D21S236. |
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| Remarks |
Line GA9-3; human fibroblasts, GM08210, 45,XX,-4,-21,+t(4;21)(p16.3;q11.2) fused with CHO K1 mutant Ade A cells; grown in hypoxanthine-free HF12 medium with 7% dialyzed FBS; retains der(4)t(4;21) in 96% of cells & other human chromosomes |
| Chastain-Moore AM, Roberts T, Trott DA, Newbold RF, Ornelles DA, An activity associated with human chromosome 21 permits nuclear colocalization of the adenovirus E1B-55K and E4orf6 proteins and promotes viral late gene expression. J Virol77(14):8087-98 2003 |
| PubMed ID: 12829847 |
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| Yu J, Hartz J, Xu Y, Gemmill RM, Korenberg JR, Patterson D, Kao FT, Isolation, characterization, and regional mapping of microclones from a human chromosome 21 microdissection library. Am J Hum Genet51:263-72 1992 |
| PubMed ID: 1642227 |
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| Gardiner K, Watkins P, Munke M, Drabkin H, Jones C, Patterson D, Partial physical map of human chromosome 21. Somat Cell Mol Genet14:623-37 1988 |
| PubMed ID: 3194801 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F12 with 2mM L-glutamine or equivalent |
| Serum |
7% dialyzed fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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