GM11978
Somatic cell hybrid
Description:
CHROMOSOME 21 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 10 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Human/Chinese hamster somatic cell hybrid retains a human der(21)t(10;21) (q24.1;q22.1) in 92% of cells
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Line 9542C-5a; fusion of human lymphos, GM09542, 46,XY,t(10;21)(10pter>10q24.1:: 21q22.1>21qter;21pter>21q22.1::10q24.1> 10qter), & Chinese hamster ovary-K1 purine auxotrophic mutant AdeC cells; grown in hypoxanthine-free HF12 med & 5% dialyzed FBS; der(21)t(10;21)(q24.1;q22.1) in 92% of cells |
| Gardiner K, Watkins P, Munke M, Drabkin H, Jones C, Patterson D, Partial physical map of human chromosome 21. Somat Cell Mol Genet14:623-37 1988 |
| PubMed ID: 3194801 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F12 deficient Medium with 2mM L-glutamine or equivalent |
| Serum |
5% dialyzed fetal bovine serum |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Proline 50mg/ml stock |
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