GM11944
Somatic cell hybrid
Description:
CHROMOSOME 11 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Chinese hamster/human hybrid retaining human 11pter>cen translocated to a hamster chromosome
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 2p25, D2S1, and 2q37, D2S3. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 4 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 4p16.2p15.1, D4S20. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 11 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 11p15.5, D11S12, and gave a negative result with a probe for 11q23.3, ETS1. PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 11p13, CAT, and gave a negative result with a primer for 11q21-q22, CLG. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 16 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 16q13q22.1, MT2, and 16pterp13, D16S36. |
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| Remarks |
Line J1-11; produced by the fusion of normal human amniotic fluid cells with Chinese hamster gly-A auxotrophic mutant cells; grown without selection; retains human 11pter>cent translocated to a hamster chromosome in 68% of cells |
| O'Keefe DS, Bacich DJ, Heston WD, Comparative analysis of prostate-specific membrane antigen (PSMA) versus a prostate-specific membrane antigen-like gene The Prostate58:200-10 2004 |
| PubMed ID: 14716746 |
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| Prawitt D, Enklaar T, Klemm G, Gartner B, Spangenberg C, Winterpacht A,
Higgins M, Pelletier J, Zabel B, Identification and characterization of MTR1, a novel gene with homology to
melastatin (MLSN1) and the trp gene family located in the BWS-WT2 critical
region on chromosome 11p15.5 and showing allele-specific expression. Curr Biol9(2):203-16 2000 |
| PubMed ID: 10607831 |
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| Gabriel JM, Higgins MJ, Gebuhr TC, Shows TB, Saitoh S, Nicholls RD, A model system to study genomic imprinting of human genes. Proc Natl Acad Sci U S A95:14857-62 1998 |
| PubMed ID: 9843980 |
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| O'Keefe DS, Su SL, Bacich DJ, Horiguchi Y, Luo Y, Powell CT, Zandvliet D, Russell PJ, Molloy PL, Nowak NJ, Shows TB, Mullins C, Vonder Haar RA, Fair WR, Heston WDW, Mapping, genomic organization and promoter analysis of the human prostate-specific membrane antigen gene [In Process Citation] Biochim Biophys Acta1443:113-27 1998 |
| PubMed ID: 9838072 |
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| Leek J, Lench N, Maraj B, Bailey A, Carr IM, Andersen S, Cross J, Whelan P, MacLennan KA, Meredith DM, Prostate-specific membrane antigen: evidence for the existence of a second related human gene British journal of cancer72:583-8 1995 |
| PubMed ID: 7669565 |
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| Tokino T, Takahashi E, Mori M, Tanigami A, Glaser T, Park JW, Jones C, Hori T, Nakamura Y, Isolation and mapping of 62 new RFLP markers on human chromosome 11. Am J Hum Genet48:258-68 1991 |
| PubMed ID: 1671318 |
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| Glaser T, Housman D, Lewis WH, Gerhard D, Jones C, A fine-structure deletion map of human chromosome 11p: analysis of J1 series hybrids. Somat Cell Mol Genet15:477-501 1989 |
| PubMed ID: 2595451 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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