GM11939
Somatic cell hybrid
Description:
CHROMOSOME 11 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Mouse/human hybrid retaining human der(16)t(11;16), #8, 9, 13, 14, 15, 17, 19, 20, 21, & 22
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 1 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 1p36, PND. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 11 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 11p13, CAT, and gave a negative result with a probe for 11q23.3, ETS1. PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 11p13, CAT, and gave a negative result with a primer for 11q21-q22, CLG. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 13 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 13q34, D13S3. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 16 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 16q13q22.1, MT2, and gave a weakly positive result with a probe for 16pterp13, D16S36. |
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| Remarks |
Line 16TX2; product of the fusion of human lymphoblasts, 46,XX,t(11;16)(p11.2; p11.1), GM06316A, with HPRT-deficient mouse myeloma P3/NS-1 cells; grown without selection; retains human der(16)t(11;16) in 76% of cells and other human chromosomes |
| Leek J, Lench N, Maraj B, Bailey A, Carr IM, Andersen S, Cross J, Whelan P, MacLennan KA, Meredith DM, Prostate-specific membrane antigen: evidence for the existence of a second related human gene British journal of cancer72:583-8 1995 |
| PubMed ID: 7669565 |
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| Junien C, van Heyningen V, Evans G, Little P, Mannens M, Report of the second chromosome 11 workshop. Genomics12:620-5 1992 |
| PubMed ID: 1348491 |
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| Seawright A, Fletcher JM, Fantes JA, Morrison H, Porteous DJ, Li SS, Hastie ND, Van Heyningen V, Analysis of WAGR deletions and related translocations with gene- specific DNA probes, using FACS-selected cell hybrids. Somat Cell Mol Genet14:21-30 1988 |
| PubMed ID: 2829363 |
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| Spurr NK, Gough AC, Gosden J, Rout D, Porteous DJ, van Heyningen V, Docherty AJ, Restriction fragment length polymorphism analysis and assignment of the metalloproteinases stromelysin and collagenase to the long arm of chromosome 11. Genomics2:119-27 1988 |
| PubMed ID: 2900807 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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