GM11766
Somatic cell hybrid
Description:
CHROMOSOME 13 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and In situ hybridization
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ISCN
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Chinese hamster/human hybrid retaining human del(13) & #6, 19, & 21
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - Y CHROMOSOME |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for Yq11, DYS227. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 13 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 13q14.3, RB1, 13q11-q12, D13S232, and 13q34, F7.1, and gave a negative result with a primer for 13q22-q31, D13S317. |
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| Remarks |
Line GS89a; produced by the fusion of human lymphos, GM07941, 46,XX,del(13) (pter>q14.3::q31>qter) with Chinese hamster ovary UV-135 cells; grown in medium without selection; retains human del(13) in 80% of cells & other human chromos |
| Warburton D, Yu MT, Tantravahi U, Lee C, Cayanis E, Russo J, Fischer SG, Regional localization of 32 NotI-HindIII fragments from a human chromosome 13 library by a somatic cell hybrid panel and in situ hybridization. Genomics16:355-60 1993 |
| PubMed ID: 8314575 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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