GM11751
Somatic cell hybrid
Description:
CHROMOSOME 3 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Human/Chinese hamster hybrid retaining human 3qter>3q11 translocated to a hamster chromosome
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 3p25, RAF1, and gave a positive result with a probe for 3q26.2qter, TFRC. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 3p14.1-p12, D3S1566, 3p22-p21.33, GLB1, and 3p25-p24.2, D3S1304, and gave positive results with primers for 3q13.1-q13.2, D3S1310, 3q22-q24, D3S1764, 3q26.2-q27, GLUT2, and 3q28q29, D3S1311. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 22q12.3q13.1, PDGFB. |
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| Remarks |
Line UCH12; produced by the fusion of human cells with CHO Urd-C cells; grown in HF12 medium with 5% dialyzed FBS; retains human 3q11>qter translocated to a hamster chromosome in 96% of cells |
| Jones MH, Zhang Y, Tirosvoutis KN, Davey PM, Webster AR, Walsh D, Spurr NK, Affara NA, Chromosomal assignment of 311 sequences transcribed in human adult testis. Genomics40:155-67 1997 |
| PubMed ID: 9070934 |
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| Ayyanathan K, Naylor SL, Kunapuli SP, Structural characterization and fine chromosomal mapping of the human P2Y1 purinergic receptor gene (P2RY1). Somat Cell Mol Genet22:419-24 1996 |
| PubMed ID: 9039850 |
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| Bailey A, Norris AL, Leek JP, Clissold PM, Carr IM, Ogilvie DJ, Morrison JF, Meredith DM, Markham AF, Yeast artificial chromosome cloning of the beta-catenin locus on human chromosome 3p21-22. Chromosome Res3:201-3 1995 |
| PubMed ID: 7780664 |
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| Hess JF, Casselman JT, FitzGerald PG, Chromosomal locations of the genes for the beaded filament proteins CP 115 and CP 47. Curr Eye Res14:11-8 1995 |
| PubMed ID: 7720401 |
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| Naylor SL, Buys CH, Carritt B, Report and abstracts of the Fourth International Workshop on Human Chromosome 3 Mapping Cytogenetics and cell genetics65:2-50 1994 |
| PubMed ID: 8104766 |
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| Drabkin H, Wright M, Jonsen M, Varkony T, Jones C, Sage M, Gold S, Morse H, Mendez M, Erickson P, Development of a somatic cell hybrid mapping panel and molecular probes for human chromosome 3. Genomics8:435-46 1990 |
| PubMed ID: 2286371 |
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| Gerber MJ, Drabkin HA, Firnhaber C, Miller YE, Scoggin CH, Smith DI, Regional localization of chromosome 3-specific DNA fragments by using a hybrid cell deletion mapping panel. Am J Hum Genet43:442-51 1988 |
| PubMed ID: 2902784 |
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| Miller YE, Daniels GL, Jones C, Palmer DK, Identification of a cell-surface antigen produced by a gene on human chromosome 3 (cen-q22) and not expressed by Rhnull cells. Am J Hum Genet41:1061-70 1987 |
| PubMed ID: 3120581 |
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| Patterson D, Jones C, Morse H, Rumsby P, Miller Y, Davis R, Structural gene coding for multifunctional protein carrying orotate phosphoribosyltransferase and OMP decarboxylase activity is located on long arm of human chromosome 3. Somatic Cell Genet9:359-74 1983 |
| PubMed ID: 6574608 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F12 with 2mM L-glutamine or equivalent |
| Serum |
5% dialyzed fetal bovine serum |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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