GM11623
Somatic cell hybrid
Description:
CHROMOSOME 8 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and In situ hybridization
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ISCN
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Chinese hamster/human hybrid retaining human 8p22>8cen & other human chromosomes
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 8 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 8q22q23, D8S5, and 8q13q22.1, CA2, and gave a positive result with a probe for 8p21, NEFL. M. Wagner, Research Triangle Park, NC, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 8p22, D8S258, and 8p21.3-cen, D8S259, and gave negative results with primers for 8q11, MOS, 8q13q22.1, D8S286, 8q22.2, D8S271, 8q22.3, D8S276, and 8q24.3-qter, D8S263. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 8p12, D8S87, and 8p21-p11.2, LHRH, and gave negative results with primers for 8p23-p22, DEF1, 8q11.22-q12, D8S260, 8q22.2, D8S270, 8q23-q24, D8S199, and 8q24, TG. |
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| Remarks |
Line 20XP0435-2; human/Chinese hamster ovary somatic cell hybrid retaining human 8p22>cen inserted between rodent chromosome segments in 95% of cells and other human chromosome material |
| White RA, Dowler LL, Pasztor LM, Gatson LL, Adkison LR, Angeloni SV, Wilson DB, Assignment of the transcription factor GATA4 gene to human chromosome 8 and mouse chromosome 14: Gata4 is a candidate gene for Ds (disorganization). Genomics27:20-6 1995 |
| PubMed ID: 7665171 |
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| Wagner MJ, Ge Y, Siciliano M, Wells DE, A hybrid cell mapping panel for regional localization of probes to human chromosome 8. Genomics10:114-25 1991 |
| PubMed ID: 2045096 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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