GM11449
Somatic cell hybrid
Description:
CHROMOSOME 4 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 12 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and In situ hybridization
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ISCN
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Chinese hamster/human hybrid retaining a der(12)t(4;12) in 100% of cells; 15% of cells had human chromosome fragments
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for Xp22.32 and Yp11.3, MIC2. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 4 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 4p16.2p15.1, D4S20, and 4q21q23, ADH3. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 4p15.3, QDPR, 4p16.3, D4S126, 4p16.3, PDEB.2, and 4q25, EGF, and gave positive results with primers for 4q28, FGA, 4q31-q33, D4S1626, and 4q35, F11. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 7 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 7pterp15, ASSP11, and 7q31.1q31.2, D7S18. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 12 |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 12p13.2, PRB1, and 12q14q24.1, D12S7. |
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| Remarks |
Line HHW582; human/Chinese hamster somatic cell hybrid retaining a human der(12)t(4;12)(12qter>12p13::4q25>4qter) in 100% of cells; grown in medium containing hypoxanthine & thymidine at 39C |
| Alkhayat AH, Kraemer SA, Leipprandt JR, Macek M, Kleijer WJ, Friderici KH, Human beta-mannosidase cDNA characterization and first identification of a mutation associated with human beta-mannosidosis. Hum Mol Genet7:75-83 1998 |
| PubMed ID: 9384606 |
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| Liang Y, Chen H, Asher JH Jr, Chang CC, Friedman TB, Human inner ear OCP2 cDNA maps to 5q22-5q35.2 with related sequences on chromosomes 4p16.2-4p14, 5p13-5q22, 7pter-q22, 10 and 12p13-12qter. Gene184:163-7 1997 |
| PubMed ID: 9031623 |
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| Inazawa J, Ariyama T, Abe T, Druck T, Ohta M, Huebner K, Yanagisawa J, Reed JC, Sato T, PTPN13, a fas-associated protein tyrosine phosphatase, is located on the long arm of chromosome 4 at band q21.3. Genomics31:240-2 1996 |
| PubMed ID: 8824809 |
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| Anderson DM, Johnson L, Glaccum MB, Copeland NG, Gilbert DJ, Jenkins NA, Valentine V, Kirstein MN, Shapiro DN, Morris SW, et al, Chromosomal assignment and genomic structure of Il15. Genomics25:701-6 1995 |
| PubMed ID: 7759105 |
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| Slaugenhaupt SA, Roca AL, Liebert CB, Altherr MR, Gusella JF, Reppert SM, Mapping of the gene for the Mel1a-melatonin receptor to human chromosome 4 (MTNR1A) and mouse chromosome 8 (Mtnr1a). Genomics27:355-7 1995 |
| PubMed ID: 7558006 |
| Split Ratio |
1:5 |
| Temperature |
39 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Hypoxanthine 5 x 10-5M |
| Supplement |
Thymidine 1 x 10-5M |
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