GM11445
Somatic cell hybrid
Description:
CHROMOSOME 5 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 6 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Human/Chinese hamster hybrid retaining a human der(5)t(5;6), #8, & #21
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5 |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 5pterp15, D5S10, and 5q33q35, PDGFRB. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 5p14-p12, C9, and 5q13.3-q14, HMGCR. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 6 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 6pterp24, D6S7. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 6p21.3, TNFA, 6p25, D6S344, and 6q24-q27, MAS1, and gave a positive result with a primer for 6q25.2-q27, D6S297. |
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| Remarks |
Line HHW1138; human/Chinese hamster somatic cell hybrid; retains human der(5) t(5;6)(q33.3;q25.3) in 100% of cells, #8 in 97% of cells, & #21 in 94% of cells; grown in culture medium containing 0.2mM proline at 39C |
| Nagarajan L, Zavadil J, Claxton D, Lu X, Fairman J, Warrington JA, Wasmuth JJ, Chinault AC, Sever CE, Slovak ML, et al, Consistent loss of the D5S89 locus mapping telomeric to the interleukin gene cluster and centromeric to EGR-1 in patients with 5q- chromosome. Blood83:199-208 1994 |
| PubMed ID: 8274735 |
| Split Ratio |
1:5 |
| Temperature |
39 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Proline 0.2 mM |
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