GM11250
Somatic cell hybrid
Description:
CHROMOSOME 15 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Human/mouse hybrid retaining human der(17)t(15;17) in 80% of cells, #15 (8%), #17 (94%), & other human chromos
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - Y CHROMOSOME |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for Yq11, DYS227. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 15 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 15q21q22.2, B2M, and 15q23, D15S1485A, and gave negative results with primers for 15q11-q12, D15S11, and 15q11.2-q12, GABRB3. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 17p13.1, MYH2, and 17q21.3q23, MPO. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 17p13.1, TP53, 17q21.3-q23, MPO1, 17q, GAS, and 17q25-qter, D17S784. |
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| Remarks |
Line LS-6; produced by the fusion of cells from a pt with Prader-Willi syndrome, t(15;17)(q13;p13.3), with LMTKmouse cells; retains the human der(17) in 80% of cells, #17 (94%), & other human chromosomes; grown in HAT medium |
| van Tuinen P, Rich DC, Summers KM, Ledbetter DH, Regional mapping panel for human chromosome 17: application to neurofibromatosis type 1. Genomics1:374-81 1987 |
| PubMed ID: 3130306 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
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