GM11102
Somatic cell hybrid
Description:
CHROMOSOME 16 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 3 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Mouse/human hybrid retaining a human der(3)t(3;16) & #10
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 3p25, RAF1, and gave a negative result with a probe for 3q26.2qter, TFRC. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 3p14.1-p12, D3S1566, 3p22-p21.33, GLB1, and 3p25-p24.2, D3S1304, and gave negative results with primers for 3q13.1-q13.2, D3S1310, 3q22-q24, D3S1764, 3q26.2-q27, GLUT2, and 3q28q29, D3S1311. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 16 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 16pterp13, D16S36, and gave a positive result with a probe for 16q13q22.1, MT2. PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 16p13.3, HBA, and gave positive results with primers for 16q22.1, LCAT, 16q23.2, D16S266, 16q24.2, D16S389, 16q24.3, D16S305, and 16q24.3, PRSM1. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 22q12.3q13.1, PDGFB. |
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| Remarks |
Line CY7; cloned; produced by the fusion of human fibro, GM02860, 46,XY,t(3;16) (q13.2;q13), with APRT- & HPRT-def mouse A9 cells; grown in AAT medium; retains human der(3)t(3;16) in 72% of cells & #10 in 68% of cells |
| Argeson AC, Druck T, Veronese ML, Knopf JL, Buchberg AM, Huebner K, Siracusa LD, Phospholipase C gamma-2 (Plcg2) and phospholipase C gamma-1 (Plcg1) map to distinct regions in the human and mouse genomes. Genomics25:29-35 1995 |
| PubMed ID: 7774933 |
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| Bailey A, Norris AL, Leek JP, Clissold PM, Carr IM, Ogilvie DJ, Morrison JF, Meredith DM, Markham AF, Yeast artificial chromosome cloning of the beta-catenin locus on human chromosome 3p21-22. Chromosome Res3:201-3 1995 |
| PubMed ID: 7780664 |
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| Callen DF, Doggett NA, Stallings RL, Chen LZ, Whitmore SA, Lane SA, Nancarrow JK, Apostolou S, Thompson AD, Lapsys NM, et al, High-resolution cytogenetic-based physical map of human chromosome 16. Genomics13:1178-85 1992 |
| PubMed ID: 1505951 |
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| Sieburth D, Jabs EW, Warrington JA, Li X, Lasota J, LaForgia S, Kelleher K, Huebner K, Wasmuth JJ, Wolf SF, Assignment of genes encoding a unique cytokine (IL12) composed of two unrelated subunits to chromosomes 3 and 5. Genomics14:59-62 1992 |
| PubMed ID: 1358798 |
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| Callen DF, Baker E, Eyre HJ, Lane SA, An expanded mouse-human hybrid cell panel for mapping human chromosome 16. Ann Genet33:190-5 1990 |
| PubMed ID: 2095701 |
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| Callen DF, Hyland VJ, Baker EG, Fratini A, Simmers RN, Mulley JC, Sutherland GR, Fine mapping of gene probes and anonymous DNA fragments to the long arm of chromosome 16. Genomics2:144-53 1988 |
| PubMed ID: 2900808 |
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| Callen DF, A mouse-human hybrid cell panel for mapping human chromosome 16. Ann Genet29:235-9 1986 |
| PubMed ID: 3495225 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F12 with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Adenine 7.5 x 10-5M |
| Supplement |
Aminopterin 1.6 x 10-6M |
| Supplement |
Thymidine 3.2 x 10-5M |
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