GM11099
Somatic cell hybrid
Description:
CHROMOSOME 16 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME X REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and Southern blot hybridization
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ISCN
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Human/mouse somatic cell hybrid retaining human der(X)t(X;16) chromosome
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for Xp22.32 (also Yp11.3), MIC2, and gave a negative result with a probe for Xq28, DXS15. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for Xq26, HPRT and Xq28, G6PD, and gave positive results with primers for Xp11.21, ALAS2, Xp11.3, DXS1003, Xp22.32, STS, Xq12, DXS453, Xq13.3, PGK1, and Xq21.3, DXS3. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 16 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 16pterp13, D16S36, and 16q13q22.1, MT2. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 16p13.3, HBA, 16q22.1, LCAT, and 16q23.2, D16S266, and gave a positive result with a primer for 16q24.3, PRSM1. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 17q21.3q23, MPO. |
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| Remarks |
Line CY2; produced by the fusion of human fibro, GM03884, 46,X,t(X;16)(q26; q24), with APRT- & HPRT- deficient mouse A9 cells; selected in alanosine-adenine medium; grown in AAT medium; retains human der(X)t(X;16) in 100% of cells |
| Pilia G, Hughes-Benzie RM, MacKenzie A, Baybayan P, Chen EY, Huber R, Neri G, Cao A, Forabosco A, Schlessinger D, Mutations in GPC3, a glypican gene, cause the Simpson-Golabi-Behmel overgrowth syndrome [see comments] Nat Genet12:241-7 1996 |
| PubMed ID: 8589713 |
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| Oaks MK, Hanson JP Jr, O'Malley DP, Molecular cytogenetic mapping of the human melanoma antigen (MAGE) gene family to chromosome region Xq27-qter: implications for MAGE immunotherapy. Cancer Res54:1627-9 1994 |
| PubMed ID: 8137270 |
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| Callen DF, Doggett NA, Stallings RL, Chen LZ, Whitmore SA, Lane SA, Nancarrow JK, Apostolou S, Thompson AD, Lapsys NM, et al, High-resolution cytogenetic-based physical map of human chromosome 16. Genomics13:1178-85 1992 |
| PubMed ID: 1505951 |
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| Callen DF, Baker E, Eyre HJ, Lane SA, An expanded mouse-human hybrid cell panel for mapping human chromosome 16. Ann Genet33:190-5 1990 |
| PubMed ID: 2095701 |
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| Callen DF, Hyland VJ, Baker EG, Fratini A, Simmers RN, Mulley JC, Sutherland GR, Fine mapping of gene probes and anonymous DNA fragments to the long arm of chromosome 16. Genomics2:144-53 1988 |
| PubMed ID: 2900808 |
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| Callen DF, A mouse-human hybrid cell panel for mapping human chromosome 16. Ann Genet29:235-9 1986 |
| PubMed ID: 3495225 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F12 with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Adenine 7.5 x 10-5M |
| Supplement |
Aminopterin 1.6 x 10-6M |
| Supplement |
Thymidine 3.2 x 10-5M |
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