GM10156
Somatic cell hybrid
Description:
NIGMS MAPPING PANEL #2, VERSION 3, HUMAN/RODENT SOMATIC CELL HYBRIDS
NIGMS HUMAN/RODENT SOMATIC CELL HYBRID MAPPING PANEL #2 DNA
NIGMS HUMAN/RODENT SOMATIC CELL HYBRID MINI MAPPING PANEL #2 DNA
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Human/Chinese hamster hybrid retaining human chromosome #8 (1 copy) in 60% of cells & 2 copies in 40% of cells
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for Xp22.32, STS, Xq13.3, PGK1, and Xq28, G6PD. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 1 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 1p13, NGFB, and 1q32, REN. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 2p13, TGFA, 2p23, POMC, and 2q13, IL1A. PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 2q37, ALPP. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 3p22-p21.33, GLB1, and 3q26.2-q27, GLUT2. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 4 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 4p15.3, QDPR, and 4q11-q13, AFP. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 5p14-p12, C9, and 5q13.3-q14, HMGCR. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 6 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 6pterp24, D6S7, and 6q24q27, ESR. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 6p21.3, TNFA, and 6q24-q27, MAS1. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 7 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 7q31q32, D7S347, and 7pterp15, ASSP11. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 7p12, EGFR, and 7q31,3, CFTR. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 8 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 8p22p12, NFL. M. Wagner, University of Houston, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 8p21-p11.2, LHRH. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 8p23-p22, DEF1, and 8q24, TG, and gave a negative result with a primer for 8p21-p11.2, LHRH. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 8p12, D8S87, 8p21-p11.2, LHRH, 8p23-p22, DEF1, 8q11.22-q12, D8S260, 8q22.2, D8S270, and 8q24, TG. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 9 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 9p22, IFNA, and 9q22.3-q31, ALDOB. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 9p21, D9S270, 9pter-p22, D9S178, and 9pter-q12, RLN1. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 10 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 10p11.2. ITGB1, and 10q24, PLAU. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 11 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 11p13, CAT, and 11q21-q22, CLG. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 12 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 12p13.3-p13.2, F8VWF, and 12q22-q24.2, PAH. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 13 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 13q34, D13S3. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 13q14.3, RB1. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 14 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 14q11.2, NP, and 14q32.1, PI. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 15 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 15q21-q22.2, B2M. PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 15q11.2-q12, GABRB3. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 16 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 16p13.3, HBA, 16q22.1, HPR, and 16q22.1, LCAT. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 17p13.1, TP53, 17q, GAS, and 17q21.3-q23, MPO. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 18 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 18q12.3, D18S6, and 18q23, MBP. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 18p11.31-p11.22, TYMS. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 18p11.1-q11.2, D18S44, and 18q12.1, TTR. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 19 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 19q13.3, LHB, and 19p13.1, D19S11. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 19p13.2, LDLR, and 19q13.2, APOC2. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 20 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 20pter-p12, PRNP, and 20q12-q13.11, ADA. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 21 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 21q22.3, ITGB2. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 22q11.2, IGLC2. |
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| Remarks |
Line 706B6, clone 17; normal human lymphocytes fused with Chinese hamster ovary cells (Gly-B, Ade-E mutant); selected in glycine-free F12 medium with 5% macroserum (dialyzed); retains intact human chromosome #8 in 100% of cells |
| Krona C, Ejeskär K, Carén H, Abel F, Sjöberg RM, Martinsson T, A novel 1p362 located gene, APITD1, with tumour-suppressive properties and a putative p53-binding domain, shows low expression in neuroblastoma tumours British journal of cancer91:1119-30 2004 |
| PubMed ID: 15328517 |
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| Mallet F, Bouton O, Prudhomme S, Cheynet V, Oriol G, Bonnaud B, Lucotte G, Duret L, Mandrand B, The endogenous retroviral locus ERVWE1 is a bona fide gene involved in hominoid placental physiology Proceedings of the National Academy of Sciences of the United States of America101:1731-6 2004 |
| PubMed ID: 14757826 |
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| Conte C, Ebeling M, Marcuz A, Nef P, Andres-Barquin PJ, Identification and characterization of human taste receptor genes belonging to the TAS2R family Cytogenetic and genome research98:45-53 2003 |
| PubMed ID: 12584440 |
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| Hansford LM, Smith SA, Haber M, Norris MD, Cheung B, Marshall GM, Cloning and characterization of the human neural cell adhesion molecule, CNTN4 (alias BIG-2) Cytogenetic and genome research101:17-23 2003 |
| PubMed ID: 14571131 |
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| Janssens B, Mohapatra B, Vatta M, Goossens S, Vanpoucke G, Kools P, Montoye T, van Hengel J, Bowles NE, van Roy F, Towbin JA, Assessment of the CTNNA3 gene encoding human alpha T-catenin regarding its involvement in dilated cardiomyopathy Human genetics112:227-36 2003 |
| PubMed ID: 12596047 |
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| Tarafa G, Prat E, Risques RA, González S, Camps J, Grau M, Guinó E, Moreno V, Esteller M, Herman JG, Germà JR, Miró R, Peinado MA, Capellá G, Common genetic evolutionary pathways in familial adenomatous polyposis tumors Cancer research63:5731-7 2003 |
| PubMed ID: 14522893 |
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| Anderson MJ, Viars CS, Czekay S, Cavenee WK, Arden KC, Cloning and characterization of three human forkhead genes that comprise an FKHR-like gene subfamily. Genomics47:187-99 1998 |
| PubMed ID: 9479491 |
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| Jones MH, Zhang Y, Tirosvoutis KN, Davey PM, Webster AR, Walsh D, Spurr NK, Affara NA, Chromosomal assignment of 311 sequences transcribed in human adult testis. Genomics40:155-67 1997 |
| PubMed ID: 9070934 |
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| Schwarz H, Arden K, Lotz M, CD137, a member of the tumor necrosis factor receptor family, is located on chromosome 1p36, in a cluster of related genes, and colocalizes with several malignancies Biochemical and biophysical research communications235:699-703 1997 |
| PubMed ID: 9207223 |
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| Borrow J, Stanton VP Jr, Andresen JM, Becher R, Behm FG, Chaganti RS, Civin CI, Disteche C, Dube I, Frischauf AM, Horsman D, Mitelman F, Volinia S, Watmore AE, Housman DE, The translocation t(8;16)(p11;p13) of acute myeloid leukaemia fuses a putative acetyltransferase to the CREB-binding protein [see comments] Nat Genet14:33-41 1996 |
| PubMed ID: 8782817 |
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| Hirai M, Kusuda J, Hashimoto K, Assignment of human ADP ribosylation factor (ARF) genes ARF1 and ARF3 to chromosomes 1q42 and 12q13, respectively. Genomics34(2):263-5 1996 |
| PubMed ID: 8661066 |
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| Arden KC, Viars CS, Weiss S, Argentin S, Nemer M, Localization of the human B-type natriuretic peptide precursor (NPPB) gene to chromosome 1p36. Genomics26:385-9 1995 |
| PubMed ID: 7601467 |
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| Bermingham JR Jr, Arden KC, Naumova AK, Sapienza C, Viars CS, Fu XD, Khotz J, Manley JL, Rosenfeld MG, Chromosomal localization of mouse and human genes encoding the splicing factors ASF/SF2 (SFRS1) and SC-35 (SFRS2). Genomics29:70-9 1995 |
| PubMed ID: 8530103 |
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| White RA, Dowler LL, Pasztor LM, Gatson LL, Adkison LR, Angeloni SV, Wilson DB, Assignment of the transcription factor GATA4 gene to human chromosome 8 and mouse chromosome 14: Gata4 is a candidate gene for Ds (disorganization). Genomics27:20-6 1995 |
| PubMed ID: 7665171 |
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| Chang DY, Nelson B, Bilyeu T, Hsu K, Darlington GJ, Maraia RJ, A human Alu RNA-binding protein whose expression is associated with accumulation of small cytoplasmic Alu RNA. Mol Cell Biol14:3949-59 1994 |
| PubMed ID: 8196634 |
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| Driscoll CT, Darlington GJ, Maraia RJ, The conserved 7SK snRNA gene localizes to human chromosome 6 by homolog
exclusion probing of somatic cell hybrid RNA. Nucleic Acids Res22(5):722-5 1994 |
| PubMed ID: 8139910 |
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| Maraia RJ, Sasaki-Tozawa N, Driscoll CT, Green ED, Darlington GJ, The human Y4 small cytoplasmic RNA gene is controlled by upstream elements and resides on chromosome 7 with all other hY scRNA genes Nucleic acids research22:3045-52 1994 |
| PubMed ID: 7520568 |
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| Weiss J, Rubinfeld B, Polakis PG, McCormick F, Cavenee WK, Arden KC, The RAP1GA1 locus for human Rap1-GTPase activating protein 1 maps to chromosome 1p36.1-->p35. Cytogenet Cell Genet66:18-21 1994 |
| PubMed ID: 8275700 |
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| Drwinga HL, Toji LH, Kim CH, Greene AE, Mulivor RA, NIGMS human/rodent somatic cell hybrid mapping panels 1 and 2. Genomics16:311-4 1993 |
| PubMed ID: 8314568 |
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| Maraia RJ, Driscoll CT, Bilyeu T, Hsu K, Darlington GJ, Multiple dispersed loci produce small cytoplasmic Alu RNA. Mol Cell Biol13:4233-41 1993 |
| PubMed ID: 7686619 |
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| Drabkin HA, Diaz M, Bradley CM, Le Beau MM, Rowley JD, Patterson D, Isolation and analysis of the 21q+ chromosome in the acute myelogenous leukemia 8;21 translocation: evidence that c-mos is not translocated. Proc Natl Acad Sci U S A82:464-8 1985 |
| PubMed ID: 2982159 |
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| Jones C, Patterson D, Kao FT, Assignment of the gene coding for phosphoribosylglycineamide formyltransferase to human chromosome 14. Somatic Cell Genet7:399-409 1981 |
| PubMed ID: 7197058 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F12 deficient Medium with 2mM L-glutamine or equivalent |
| Serum |
7% dialyzed fetal bovine serum |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Hypoxanthine 3 x 10-5M |
| Supplement |
Proline 50mg/ml |
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