GM10063
Somatic cell hybrid
Description:
CHROMOSOME X REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 21 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and Southern blot hybridization
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ISCN
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Mouse/human hybrid retaining a human der(X)t(X;21)(p21;p12)
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for Xp22.2, DXS16, and Xp22.32 (also Yp11.3), MIC2, and a positive result with a probe for Xq28, DXS15. PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for Xp22.32, STS, and gave positive results with primers for Xp11.21, ALAS2, Xp11.3, DXS1003, Xq12, DXS453, Xq13.3, PGK1, Xq21.3, DXS3, Xq26, HPRT, and Xq28, G6PD. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 14 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 14q32.33, IGHG4. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 16 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 16pterp13, D16S36, and 16q13q22.1, MT2. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 17q21.3q23, MPO. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 21 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 21q11, D21S26, and 21q22.1q22.2, D21S17. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 21p11.2, D21S1278, 21q11, D21S120, 21q11.1, D21S236, 21q21.2, APP, 21q21.2, AMYLOID, and 21q22.3, ITGB2. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 22q12.3q13.1, PDGFB. |
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| Remarks |
Line A2-4; HPRT-deficient mouse A9 cells fused with fibroblasts from a patient, GM10062, t(X;21)(p21;p12), with muscular dystrophy; grown in HAT medium; 98% of cells retain the human der(X) chromosome |
| van Sluis M, van Vuuren C, Mangan H, McStay B, NORs on human acrocentric chromosome p-arms are active by default and can associate with nucleoli independently of rDNA Proceedings of the National Academy of Sciences of the United States of America: 2020 |
| PubMed ID: 32332163 |
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| van Sluis M, Gailín MÓ, McCarter JGW, Mangan H, Grob A, McStay B, Human NORs, comprising rDNA arrays and functionally conserved distal elements, are located within dynamic chromosomal regions Genes & development: 2019 |
| PubMed ID: 31727772 |
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| Floutsakou I, Agrawal S, Nguyen TT, Seoighe C, Ganley AR, McStay B, The shared genomic architecture of human nucleolar organizer regions Genome research23:2003-12 2013 |
| PubMed ID: 23990606 |
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| Marshall LJ, Moore AC, Ohki M, Kitabayashi I, Patterson D, Ornelles DA, RUNX1 permits E4orf6-directed nuclear localization of the adenovirus E1B-55K protein and associates with centers of viral DNA and RNA synthesis Journal of virology82:6395-408 2008 |
| PubMed ID: 18417565 |
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| Chastain-Moore AM, Roberts T, Trott DA, Newbold RF, Ornelles DA, An activity associated with human chromosome 21 permits nuclear colocalization of the adenovirus E1B-55K and E4orf6 proteins and promotes viral late gene expression. J Virol77(14):8087-98 2003 |
| PubMed ID: 12829847 |
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| Boycott KM, Halley GR, Schlessinger D, Bech-Hansen NT, A 2-megabase physical contig incorporating 43 DNA markers on the human X chromosome at p11.23-p11.22 from ZNF21 to DXS255. Genomics33:488-97 1996 |
| PubMed ID: 8661008 |
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| Oaks MK, Hanson JP Jr, O'Malley DP, Molecular cytogenetic mapping of the human melanoma antigen (MAGE) gene family to chromosome region Xq27-qter: implications for MAGE immunotherapy. Cancer Res54:1627-9 1994 |
| PubMed ID: 8137270 |
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| Corcos IA, Lafreniere RG, Begy CR, Loch-Caruso R, Willard HF, Glover TW, Refined localization of human connexin32 gene locus, GJB1, to Xq13.1. Genomics13:479-80 1992 |
| PubMed ID: 1319395 |
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| Bodrug SE, Ray PN, Gonzalez IL, Schmickel RD, Sylvester JE, Worton RG, Molecular analysis of a constitutional X-autosome translocation in a female with muscular dystrophy. Science237:1620-4 1987 |
| PubMed ID: 3629260 |
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| Ray PN, Belfall B, Duff C, Logan C, Kean V, Thompson MW, Sylvester JE, Gorski JL, Schmickel RD, Worton RG, Cloning of the breakpoint of an X;21 translocation associated with Duchenne muscular dystrophy. Nature318:672-5 1985 |
| PubMed ID: 3001530 |
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| Worton RG, Duff C, Sylvester JE, Schmickel RD, Willard HF, Duchenne muscular dystrophy involving translocation of the dmd gene next to ribosomal RNA genes. Science224:1447-9 1984 |
| PubMed ID: 6729462 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
MEM (Eagle) Alpha Modification with nucleosides with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
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