GM09191
Somatic cell hybrid
Description:
CHROMOSOME 6 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
ADDITIONAL DELETION/DERIVATIVE X CHROMOSOME SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and In situ hybridization
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ISCN
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Mouse/Human hybrid retaining a single metacentric chromosome consistent with der(6)t(X;6)(Xqter>Xq13::6p21>6qter)
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for Xq12, DXS453, Xq13.3, PGK1, and Xq28, G6PD, and gave a negative result with a primer for Xp11.21, ALAS2. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 6 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 6q21-q23.3, D6S292, 6q24-q27, MAS1, and 6q27qter, D6S297, and gave negative results with primers for 6p21.3, TNFA, and 6p25, D6S344. |
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| Remarks |
Line MCP-6; produced by fusing mouse teratocarcinoma line PCC4-1 with human Burkitt lymphoma line G3.32.2; selected in HAT medium; retains 1 hum chromo consistent with der(6)t(X;6)(Xqter>Xq13:: 6p21>6qter) reported by the submitter |
| Oaks MK, Hanson JP Jr, O'Malley DP, Molecular cytogenetic mapping of the human melanoma antigen (MAGE) gene family to chromosome region Xq27-qter: implications for MAGE immunotherapy. Cancer Res54:1627-9 1994 |
| PubMed ID: 8137270 |
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| Corcos IA, Lafreniere RG, Begy CR, Loch-Caruso R, Willard HF, Glover TW, Refined localization of human connexin32 gene locus, GJB1, to Xq13.1. Genomics13:479-80 1992 |
| PubMed ID: 1319395 |
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| Spurr NK, Feder J, Bodmer WF, Goodfellow PN, Solomon E, Cavalli-Sforza LL, Chromosomal assignment of seven human genomic DNA sequences associated with restriction fragment length polymorphisms. Ann Hum Genet50 ( Pt 2):145-52 1986 |
| PubMed ID: 2893580 |
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| Goodfellow P, Banting G, Sheer D, Ropers HH, Caine A, Ferguson-Smith MA, Povey S, Voss R, Genetic evidence that a Y-linked gene in man is homologous to a gene on the X chromosome. Nature302:346-9 1983 |
| PubMed ID: 6188056 |
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| Goodfellow PN, Banting G, Trowsdale J, Chambers S, Solomon E, Introduction of a human X-6 translocation chromosome into a mouse teratocarcinoma: investigation of control of HLA-A, B, C expression. Proc Natl Acad Sci U S A79:1190-4 1982 |
| PubMed ID: 6951167 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
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