GM09142
Somatic cell hybrid
Description:
CHROMOSOME 21 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME X REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and Southern blot hybridization
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ISCN
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Reduced hybrid containing human chromosomes t(X;21), 3, & 5 in a mouse background
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for Xq28, DXS15, and a positive result with a probe for Xp22.3p22.2, DXS85. PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for Xp22.32, STS, and gave negative results with primers for Xp11.21, ALAS2, Xp11.3, DXS1003, Xq12, DXS453, Xq13.3, PGK1, Xq21.3, DXS3, Xq26, HPRT, and Xq28, G6PD. |
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| GENE MAPPING & DOSAGE STUDIES - Y CHROMOSOME |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for Yp11.3 (also Xp22.32), DXYS20, and Yq12, DYZ1. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 1 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 1q32, REN, and a positive result with a probe for 1p36 (also 3p21), DNF15S1/S2. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 3p25, RAF1, and 3q21, TF. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 3p22-p21.33, GLB1, and 3q26.2-q27, GLUT2. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 5q31q32, ADRBR. PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 5q13.3-q14, HMGCR. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 21 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 21q22.3, BCEI. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 21q22.3, ITGB2, 21q11, D21S120, 21q11.1, D21S236, 21q21.2, AMYLOID, and 21p11.2, D21S1278. |
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| Remarks |
Line C2-T10; HPRT-deficient A9 mouse cells fused with human fibroblasts, t(X;21)(p21;p12); 86% of cells retain the der(21), 100% have human chromosome #3, & 50% of cells have human chromosome #5 |
| van Sluis M, Gailín MÓ, McCarter JGW, Mangan H, Grob A, McStay B, Human NORs, comprising rDNA arrays and functionally conserved distal elements, are located within dynamic chromosomal regions Genes & development: 2019 |
| PubMed ID: 31727772 |
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| Floutsakou I, Agrawal S, Nguyen TT, Seoighe C, Ganley AR, McStay B, The shared genomic architecture of human nucleolar organizer regions Genome research23:2003-12 2013 |
| PubMed ID: 23990606 |
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| Chastain-Moore AM, Roberts T, Trott DA, Newbold RF, Ornelles DA, An activity associated with human chromosome 21 permits nuclear colocalization of the adenovirus E1B-55K and E4orf6 proteins and promotes viral late gene expression. J Virol77(14):8087-98 2003 |
| PubMed ID: 12829847 |
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| Anderson MJ, Viars CS, Czekay S, Cavenee WK, Arden KC, Cloning and characterization of three human forkhead genes that comprise an FKHR-like gene subfamily. Genomics47:187-99 1998 |
| PubMed ID: 9479491 |
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| Boycott KM, Halley GR, Schlessinger D, Bech-Hansen NT, A 2-megabase physical contig incorporating 43 DNA markers on the human X chromosome at p11.23-p11.22 from ZNF21 to DXS255. Genomics33:488-97 1996 |
| PubMed ID: 8661008 |
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| Ginns EI, Ott J, Egeland JA, Allen CR, Fann CS, Pauls DL, Weissenbachoff J, Carulli JP, Falls KM, Keith TP, Paul SM, A genome-wide search for chromosomal loci linked to bipolar affective disorder in the Old Order Amish. Nat Genet12:431-5 1996 |
| PubMed ID: 8630500 |
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| Arden KC, Viars CS, Weiss S, Argentin S, Nemer M, Localization of the human B-type natriuretic peptide precursor (NPPB) gene to chromosome 1p36. Genomics26:385-9 1995 |
| PubMed ID: 7601467 |
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| Argeson AC, Druck T, Veronese ML, Knopf JL, Buchberg AM, Huebner K, Siracusa LD, Phospholipase C gamma-2 (Plcg2) and phospholipase C gamma-1 (Plcg1) map to distinct regions in the human and mouse genomes. Genomics25:29-35 1995 |
| PubMed ID: 7774933 |
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| Bermingham JR Jr, Arden KC, Naumova AK, Sapienza C, Viars CS, Fu XD, Khotz J, Manley JL, Rosenfeld MG, Chromosomal localization of mouse and human genes encoding the splicing factors ASF/SF2 (SFRS1) and SC-35 (SFRS2). Genomics29:70-9 1995 |
| PubMed ID: 8530103 |
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| De Plaen E, Arden K, Traversari C, Gaforio JJ, Szikora JP, De Smet C, Brasseur F, van der Bruggen P, Lethé B, Lurquin C, Structure, chromosomal localization, and expression of 12 genes of the MAGE family Immunogenetics40:360-9 1994 |
| PubMed ID: 7927540 |
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| Kastury K, Druck T, Huebner K, Barletta C, Acampora D, Simeone A, Faiella A, Boncinelli E, Chromosome locations of human EMX and OTX genes. Genomics22:41-5 1994 |
| PubMed ID: 7959790 |
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| Oaks MK, Hanson JP Jr, O'Malley DP, Molecular cytogenetic mapping of the human melanoma antigen (MAGE) gene family to chromosome region Xq27-qter: implications for MAGE immunotherapy. Cancer Res54:1627-9 1994 |
| PubMed ID: 8137270 |
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| Weiss J, Rubinfeld B, Polakis PG, McCormick F, Cavenee WK, Arden KC, The RAP1GA1 locus for human Rap1-GTPase activating protein 1 maps to chromosome 1p36.1-->p35. Cytogenet Cell Genet66:18-21 1994 |
| PubMed ID: 8275700 |
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| Dorig RE, Marcil A, Chopra A, Richardson CD, The human CD46 molecule is a receptor for measles virus (Edmonston strain). Cell75:295-305 1993 |
| PubMed ID: 8402913 |
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| Bodrug SE, Ray PN, Gonzalez IL, Schmickel RD, Sylvester JE, Worton RG, Molecular analysis of a constitutional X-autosome translocation in a female with muscular dystrophy. Science237:1620-4 1987 |
| PubMed ID: 3629260 |
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| Ray PN, Belfall B, Duff C, Logan C, Kean V, Thompson MW, Sylvester JE, Gorski JL, Schmickel RD, Worton RG, Cloning of the breakpoint of an X;21 translocation associated with Duchenne muscular dystrophy. Nature318:672-5 1985 |
| PubMed ID: 3001530 |
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| Worton RG, Duff C, Sylvester JE, Schmickel RD, Willard HF, Duchenne muscular dystrophy involving translocation of the dmd gene next to ribosomal RNA genes. Science224:1447-9 1984 |
| PubMed ID: 6729462 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
MEM (Eagle) Alpha Modification with nucleosides with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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