AG29384

AG29384 iPSC from Fibroblast

Description:

ALZHEIMER DISEASE, FAMILIAL, TYPE 3

Affected:

Yes

Sex:

Male

Age:

35 YR (At Sampling)

Overview

Repository

NIA Aging Cell Culture Repository

Subcollection

Canadian Alzheimer Disease

Protocols

Protocol PDF

Biopsy Source

Skin

Cell Type

Stem cell

Cell Subtype

Induced pluripotent stem cell

Transformant

Reprogrammed (Sendai)

Sample Source

iPSC from Fibroblast

Race

White

Ethnicity

CANADIAN

Family Member

Confirmation

Clinical summary/Case history

ISCN

46,XY[20]

Species

Homo sapiens

Common Name

Human

Remarks

Reprogrammed from parental fibroblast AG09035. The donor has been diagnosed with Alzheimer's disease (dx updated by submitter on 6/14/2010). Same donor as AG09034 (lymphoblast). Researchers purchasing hiPSCs from the NIA Repository are responsible for any limited use label licenses (LULLs) applicable to the cell line purchased. The applicable LULL to this line is Sendai-CytoTune.

Characterizations

Passage Frozen

Induced Pluripotent Stem Cell

The parental cell line was recovered reprogrammed to an induced pluripotent stem cell line and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

Phenotypic Data

Remarks

External Links

NCBI GTR

607822 ALZHEIMER DISEASE 3; AD

OMIM

607822 ALZHEIMER DISEASE 3; AD

Omim Description

ALZHEIMER DISEASE, FAMILIAL, TYPE 3; AD3

Culture Protocols

Passage Frozen

Split Ratio

1:7

Temperature

37 C

Percent CO2

Percent O2

AMBIENT

Medium

mTeSR1

Serum

none

Substrate

Matrigel

Supplement

Pricing

Commercial:

$1,131.00USD

Academic &
Non-profit:

$823.00USD

NIA Grantees:

$412.00USD