AG22107
Fibroblast from Skin, Right ischium
Description:
NON WOUND HEALING PHENOTYPE
|
Repository
|
NIA Aging Cell Culture Repository
|
| Subcollection |
Non-Healing Wound Collection |
|
Biopsy Source
|
Right ischium
|
|
Cell Type
|
Fibroblast
|
|
Tissue Type
|
Skin
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
Fibroblast from Skin, Right ischium
|
|
Race
|
Black/African American
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
|
ISCN
|
46,XY
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| PDL at Senescence |
21 |
| PDL at Freeze |
3.97 |
| Passage Frozen |
2 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin confirmed by LINE assay |
| |
| Remarks |
Tissue sample minced for establishment of this culture was taken at debridement from a right ischial pressure wound; history of diabetes mellitus; chronic renal failure; neurogenic bladder; fourniers gangrene and multiple ulcers |
| Brem H, Golinko MS, Stojadinovic O, Kodra A, Diegelmann RF, Vukelic S, Entero H, Coppock DL, Tomic-Canic M, Primary cultured fibroblasts derived from patients with chronic wounds: a methodology to produce human cell lines and test putative growth factor therapy such as GMCSF Journal of translational medicine6:75 2008 |
| PubMed ID: 19046453 |
| PDL at Senescence |
21 |
| Cumulative PDL at Freeze |
3.97 |
| Passage Frozen |
2 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Supplement |
- |
|
|