AG22097
Fibroblast from Skin, Right ischium
Description:
NON WOUND HEALING PHENOTYPE
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Non-Healing Wound Collection |
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Biopsy Source
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Right ischium
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Right ischium
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Race
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Black/African American
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Ethnicity
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AFRICAN-AMERICAN
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Senescence |
33 |
| PDL at Freeze |
3.56 |
| Passage Frozen |
2 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin confirmed by LINE assay |
| |
| Remarks |
Tissue sample minced for establishment of this culture was taken at wound debridement from a pressure ulcer on the right ischium; patient is bedbound with bilateral ischial ulcers; multiple sclerosis |
| Brem H, Golinko MS, Stojadinovic O, Kodra A, Diegelmann RF, Vukelic S, Entero H, Coppock DL, Tomic-Canic M, Primary cultured fibroblasts derived from patients with chronic wounds: a methodology to produce human cell lines and test putative growth factor therapy such as GMCSF Journal of translational medicine6:75 2008 |
| PubMed ID: 19046453 |
| PDL at Senescence |
33 |
| Cumulative PDL at Freeze |
3.56 |
| Passage Frozen |
2 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
3% |
| Medium |
Eagles Minimum Essential Medium with Earle's salts:Dulbecco's modified MEM with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Supplement |
- |
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