AG08258
LCL from B-Lymphocyte
Description:
ALZHEIMER DISEASE; AD
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Alzheimer's Disease
Alzheimer Disease With No Family History |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Race
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Black/African American
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Remarks |
The donor had a history of progressive dementia since age 87 years. Autopsy of brain revealed numerous neurofibrillary tangles and plaques. The culture was initiated on 6/18/85 by transformation of lymphocytes with Epstein Barr virus using a post-mortem blood specimen. The cells grow in suspension and their morphology is spherical. The karyotype is 46,XY; normal diploid male. 2% of the cells examined had random chromosome loss. Same subject as AG08259 (fibroblast) and AG27711 (stem cell). The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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