NA13445
DNA from Somatic cell hybrid
Description:
CHROMOSOME 6 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 7 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
| Quantity |
0.050mg |
| Quantitation Method |
Please see our FAQ |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Sample Source
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DNA from Somatic cell hybrid
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Confirmation
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Karyotypic analysis and In situ hybridization
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ISCN
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Mouse/human hybrid retaining a human der(7)t(6;7) in 80% of cells & human #3, 10, 13, 14, 17, 19, 21, & 22
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 6 |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 6p21.3, TNFA, and 6p25, D6S344, and gave positive results with primers for 6q21-q23.3, D6S292, 6q24-q27, MAS1, and 6q27-qter, D6S297. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 7 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 7p12, EGFR, and 7pter-p22, D7S472, and gave negative results with primers for 7q31.3, CFTR, and 7q36-qter, D7S550. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 10 |
PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 10q24, PLAU. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22 |
PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 22q11.2, IGLC2. |
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| Remarks |
Line 5184-4; produced by the fusion of human lymphoblasts, GM05184, 46,XY, t(6;7)(q21;q21.2) with TK-deficient mouse Cl1D cells; selected in HAT medium; retains der(7)t(6;7) in 80% of cells & other human chromosomes |
| Pappas GJ, Polymeropoulos MH, Boyle JM, Trent JM, Regional assignment by hybrid mapping of 36 expressed sequence tags (ESTs) on human chromosome 6. Genomics25:124-9 1995 |
| PubMed ID: 7774909 |
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| Meese EU, Meltzer PS, Ferguson PW, Trent JM, Alu-PCR: characterization of a chromosome 6-specific hybrid mapping panel and cloning of chromosome-specific markers. Genomics12:549-54 1992 |
| PubMed ID: 1559706 |
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| Meese EU, Witkowski CM, Zoghbi HY, Stanbridge EJ, Meltzer PS, Trent JM, Development and utilization of a somatic cell hybrid mapping panel to assign NotI linking probes to the long arm of human chromosome 6. Genomics12:542-8 1992 |
| PubMed ID: 1559705 |
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