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GM29620 iPSC from Blood

Description:

PERSONAL GENOME PROJECT
APPARENTLY HEALTHY INDIVIDUAL

Affected:

No

Sex:

Male

Age:

49 YR (At Sampling)

  • Overview
  • Characterizations
  • Phenotypic Data
  • Culture Protocols

Overview

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Repository NIGMS Human Genetic Cell Repository
Subcollection Apparently healthy iPSCs
PIGI Consented Sample
Whole-Genome Sequence
Protocols Protocol PDF
Biopsy Source Blood
Cell Type Stem cell
Cell Subtype Induced pluripotent stem cell
Transformant Reprogrammed (Sendai)
Sample Source iPSC from Blood
Race Black/African American
Ethnicity AFRICAN-AMERICAN
Relation to Proband proband
Confirmation Clinical summary/Case history
ISCN 46,XY[20]
Species Homo sapiens
Common Name Human
Remarks Participant #10 (hu604D39) in the Personal Genome Project: http://www.personalgenomes.org Whole genome sequence data is available on the PGP website; DSP c.1dupA heterozygous, GRIA3 c.-2_-1insG homozygous, INF2 c.2310+8delA heterozygous, SPG7 c.596delG heterozygous. Researchers purchasing hiPSCs from the NIGMS Repository are responsible for any limited use label licenses (LULLs) applicable to the cell line purchased. The applicable LULL to this line is Sendai-CytoTune.

Characterizations

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Passage Frozen 15
 
Induced Pluripotent Stem Cell The parental cell line was recovered reprogrammed to an induced pluripotent stem cell line and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.
 

Phenotypic Data

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Demographic Data
Relation to Proband proband
Age at Sampling 49 YR
Sex Male
Racial Category Black/African American
 
Data Elements
Clinical Element Type: Church PHGP
  (Baseline)
Vital Signs
Allergies
Medications, Vitamins, Supplements
Medical History
Race, Ethnicity, Ancestry
Traits
Near-sighted  No Data
Far-sighted  No Data
Wear Prescription Glasses  No Data
Remarks Participant #10 (hu604D39) in the Personal Genome Project: http://www.personalgenomes.org Whole genome sequence data is available on the PGP website; DSP c.1dupA heterozygous, GRIA3 c.-2_-1insG homozygous, INF2 c.2310+8delA heterozygous, SPG7 c.596delG heterozygous. Researchers purchasing hiPSCs from the NIGMS Repository are responsible for any limited use label licenses (LULLs) applicable to the cell line purchased. The applicable LULL to this line is Sendai-CytoTune.

Culture Protocols

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Passage Frozen 15
Split Ratio 1:6
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium mTeSR1
Serum none Not inactivated
Substrate Matrigel
Supplement -
Pricing
Commercial/For-profit:
$1,789.00USD
Academic/Non-profit/Government:
$1,110.00USD
Add to Cart
How to Order
  • Ordering Instructions
  • MTA / Assurance Form
  • Statement of Research Intent Form
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