GM29371
iPSC from Fibroblast
Description:
TALEN MEDIATED INTEGRATION OF A SINGLE COPY OF CAG-DCAS9-BFP-KRAB AT THE CLYBL LOCUS AND TET-ON NGN2 AT THE AAVS1 LOCUS OF WTC-11 IPSC LINE
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Gene-Edited hiPSC |
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License Required
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For commercial institutions interested in purchasing this iPSC line, additional licensing may be required. Please reach out to our customer service team at customerservice@coriell.org for more information.
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| Protocols |
Protocol PDF |
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Cell Type
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Stem cell
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Cell Subtype
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Induced pluripotent stem cell
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Transformant
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Reprogrammed (Episomal)
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Sample Source
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iPSC from Fibroblast
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Race
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Asian
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Ethnicity
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Not Hispanic/Latino
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Ethnicity
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Japanese
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Country of Origin
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USA
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Family Member
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1
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Family History
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N
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Relation to Proband
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proband
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Confirmation
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Molecular characterization before cell line submission to CCR
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ISCN
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46,XY[19]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
8 |
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| Induced Pluripotent Stem Cell |
The frozen cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis. |
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| Remarks |
This cell line (“Original Material”) contains the Tetracycline controllable expression system (the "Tet Technology") which is covered by a series of patents including U.S. Patent Nos. 8,383,364; 9,181,556; European Patents EP 1954811; 235283 and corresponding patent claims outside these regions proprietary to TET Systems GmbH & Co. KG ("Tet Systems"). The purchaser of the Original Material (“Recipient”) is granted an automatic license to use the Tet Technology only for internal, academic research purposes, which license specifically excludes the right to sell, or otherwise transfer, the Tet Technology or its component parts to third parties.
Induced pluripotent stem cell line WTC11 derived from adult skin (leg) fibroblasts; subject clinically normal; wild-type; FISH test: 46, XY. Also available is a collection of fluorescently tagged hiPSC lines from the same subject, using CRISPR/Cas9 technology (for more information, see the Allen Cell Collection at Coriell: https://catalog.coriell.org/1/AllenCellCollection). Researchers purchasing hiPSCs from the NIGMS Repository are responsible for any limited use label licenses (LULLs) applicable to the cell line purchased. The applicable LULL to this line is iPS Academia, Japan Inc.. |
| Tian R, Gachechiladze MA, Ludwig CH, Laurie MT, Hong JY, Nathaniel D, Prabhu AV, Fernandopulle MS, Patel R, Abshari M, Ward ME, Kampmann M, CRISPR Interference-Based Platform for Multimodal Genetic Screens in Human iPSC-Derived Neurons Neuron104:239-255.e12 2019 |
| PubMed ID: 31422865 |
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| Miyaoka Y, Chan AH, Judge LM, Yoo J, Huang M, Nguyen TD, Lizarraga PP, So PL, Conklin BR, Isolation of single-base genome-edited human iPS cells without antibiotic selection Nature methods11:291-3 2013 |
| PubMed ID: 24509632 |
| Passage Frozen |
8 |
| Split Ratio |
1:6 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
mTeSR1 |
| Serum |
none |
| Substrate |
Matrigel |
| Supplement |
- |
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