GM23377
Fibroblast from Skin, Unspecified
Description:
RENAL ADYSPLASIA
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Heritable Diseases |
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Biopsy Source
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Unspecified
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Unspecified
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Race
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White
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Ethnicity
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Not Hispanic/Latino
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Family History
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N
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
3.4 |
| Passage Frozen |
1 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by LINE assay |
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| Remarks |
Stillborn at 19 weeks 6 days; bilateral renal agenesis; ultrasound at 18 fetal weeks showed abnormal kidneys, bladder and spine; anhydramnios limited anatomical survey; unilateral lower extremity limb reduction with only one left femur visible-possible sirenomelia; fetal cranium is markedly hyperextended onto the posterior cervical spine, unclear if this is due to anencephaly vs. deformation secondary to oligohydramnios; suspected splaying of the cervical vertebrae. |
| Cumulative PDL at Freeze |
3.4 |
| Passage Frozen |
1 |
| Split Ratio |
1:6 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
3% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Supplement |
- |
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