GM22651
LCL from B-Lymphocyte
Description:
MULTIPLE CELL LINES FROM SAME SUBJECT - EBV TRANSFORMED B LYMPHOCYTE
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Race
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White
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Country of Origin
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USA
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Family Member
|
1
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Family History
|
N
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Relation to Proband
|
proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin confirmed by LINE assay |
| |
| Remarks |
Clinically normal; other lymphoblast cell cultures from this same individual are GM22647, GM22648, GM22649 and GM22650 |
| Laczik M, Erdos E, Ozgyin L, Hevessy Z, Csosz É, Kalló G, Nagy T, Barta E, Póliska S, Szatmári I, Bálint BL, Extensive proteome and functional genomic profiling of variability between genetically identical human B-lymphoblastoid cells Scientific data9:763 2022 |
| PubMed ID: 36496436 |
| |
| Ozgyin L, Horvath A, Hevessy Z, Balint BL, Extensive epigenetic and transcriptomic variability between genetically identical human B-lymphoblastoid cells with implications in pharmacogenomics research Scientific reports9:4889 2018 |
| PubMed ID: 30894562 |
| |
| Scheinfeldt LB1, Hodges K2, Pevsner J3, Berlin D2, Turan N2, Gerry NP2,4., Genetic and genomic stability across lymphoblastoid cell line expansions BMC Research Notes11:4889 2018 |
| PubMed ID: 30075799 |
| |
| Shirley MD, Baugher JD, Stevens EL, Tang Z, Gerry N, Beiswanger CM, Berlin DS, Pevsner J, Chromosomal variation in lymphoblastoid cell lines Human mutation33:1075-86 2011 |
| PubMed ID: 22374857 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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