GM20913
LCL from B-Lymphocyte
Description:
SICKLE CELL ANEMIA
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Repository
|
NIGMS Human Genetic Cell Repository
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| Subcollection |
Heritable Diseases |
| Class |
Mutations of the Hemoglobin Loci |
|
Biopsy Source
|
Peripheral vein
|
|
Cell Type
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B-Lymphocyte
|
|
Tissue Type
|
Blood
|
|
Transformant
|
Epstein-Barr Virus
|
|
Sample Source
|
LCL from B-Lymphocyte
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|
Race
|
Black/African American
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|
Family Member
|
1
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
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|
Species
|
Homo sapiens
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|
Common Name
|
Human
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|
Remarks
|
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin confirmed by LINE assay |
| |
| Remarks |
Clinically healthy; sickle cell trait; hemoglobin electrophoresis results: HbA = 56.3%, HbA2 = 3.8%, HbF = 0%, HbS = 39.9%; hemoglobin = 13 g/dl; MCV = 91.6; sister is GM20914.
|
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
|
|