GM16632
Fibroblast from Skin, Unspecified
Description:
FANCONI ANEMIA, COMPLEMENTATION GROUP A; FANCA
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Heritable Diseases
Engineered Cell Cultures |
| Class |
Syndromes with Increased Chromosome Breakage |
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Biopsy Source
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Unspecified
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Unspecified
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
5.37 |
| Passage Frozen |
25 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Clinically affected; line PD720.F RV:FANCA; corrected version of complementation group A fibroblast cell line PD720.F (GM16631); corrected using pMMP-puro retroviral vector [Pulsipher M. et al Subtyping analysis of Fanconi anemia by immunoblotting and retroviral gene transfer Mol Med 4:468-479 (1998)]; cells are resistant to 1ug/ml diepoxybutane by chromosome aberration analysis. |
| Pulsipher M, Kupfer GM, Naf D, Suliman A, Lee JS, Jakobs P, Grompe M, Joenje H, Sieff C, Guinan E, Mulligan R, D'Andrea AD, Subtyping analysis of Fanconi anemia by immunoblotting and retroviral gene transfer. Mol Med4(7):468-79 1998 |
| PubMed ID: 9713825 |
| Passage Frozen |
25 |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
MEM (Eagle) Alpha Modification with nucleosides with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Supplement |
- |
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