GM12610
Fibroblast from Lung, Lung
Description:
RETICULOSIS, FAMILIAL HISTIOCYTIC
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Heritable Diseases |
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Biopsy Source
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Lung
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Cell Type
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Fibroblast
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Tissue Type
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Lung
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Lung, Lung
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
1 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Lung fibroblast culture; generalized angiocentric malignant T-lympho-proliferative process involving almost all organs, with massive involvement of the brain, lungs, liver, G I tract, spleen, and kidneys; Cushinoid body habitus and marked vascular change in adrenal cortex; renal tubular injury with calcinosis; severe thymic involution; bone marrow emboli in lungs, peri-pancreatic fat, and thyroid glands; similarly affected sister expired at age 9; 46,XX in bone marrow |
| Passage Frozen |
1 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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