GM05887
Tumor-derived cell line from Osteosarcoma
Description:
OSTEOGENIC SARCOMA
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Heritable Diseases |
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Biopsy Source
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Osteosarcoma
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Cell Type
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Tumor-derived cell line
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Tissue Type
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Tumor
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Transformant
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Murine Sarcoma Virus
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Sample Source
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Tumor-derived cell line from Osteosarcoma
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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Modal no. of 50 chromosomes; all cells XO; mean no. of chromosomes is 48; 64% are polyploid; markers 1q-,9q-,12q+,17p+
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Line 143B; human osteosarcoma cells transformed by murine sarcoma virus; deficient in thymidine kinase; tumorigenic in nude mice; passage 5 at CCR; modal no of 50 chromosomes; all cells XO; 64% are polyploid |
| Claude E, de Lhoneux G, Pierreux CE, Marbaix E, de Ville de Goyet M, Boulanger C, Van Damme A, Brichard B, Decottignies A, Detection of alternative lengthening of telomeres mechanism on tumor sections Molecular biomedicine2:32 2021 |
| PubMed ID: 35006465 |
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| Guerrero-Castillo S, Krisp C, Küchler K, Arnold S, Schlüter H, Gersting SW, Multiplexed complexome profiling using tandem mass tags Biochimica et biophysica acta Bioenergetics1862:148448 2021 |
| PubMed ID: 34015258 |
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| Ryan PA, Maher VM, McCormick JJ, Failure of infinite life span human cells from different immortality complementation groups to yield finite life span hybrids. J Cell Physiol159:151-60 1994 |
| PubMed ID: 8138583 |
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| Bhattacharyya NP, Maher VM, McCormick JJ, Intrachromosomal homologous recombination in human cells which differ in nucleotide excision-repair capacity. Mutat Res234:31-41 1990 |
| PubMed ID: 2154688 |
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| Erisman MD, Scott JK, Astrin SM, Evidence that the familial adenomatous polyposis gene is involved in a subset of colon cancers with a complementable defect in c-myc regulation. Proc Natl Acad Sci U S A86:4264-8 1989 |
| PubMed ID: 2542967 |
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| Bertholet C, Drillien R, Wittek R, One hundred base pairs of 5' flanking sequence of a vaccinia virus late gene are sufficient to temporally regulate late transcription. Proc Natl Acad Sci U S A82:2096-100 1985 |
| PubMed ID: 3856886 |
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| Croce CM, Barrick J, Linnenbach A, Koprowski H, Expression of malignancy in hybrids between normal and malignant cells. J Cell Physiol99:279-85 1979 |
| PubMed ID: 457790 |
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| Bacchetti S, Graham FL, Transfer of the gene for thymidine kinase to thymidine kinase-deficient human cells by purified herpes simplex viral DNA. Proc Natl Acad Sci U S A74:1590-4 1977 |
| PubMed ID: 193108 |
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| Rhim JS, Cho HY, Huebner RJ, Non-producer human cells induced by murine sarcoma virus. Int J Cancer15:23-9 1975 |
| PubMed ID: 165148 |
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| McAllister RM, Gardner MB, Greene AE, Bradt C, Nichols WW, Landing BH, Cultivation in vitro of cells derived from a human osteosarcoma. Cancer27:397-402 1971 |
| PubMed ID: 5100401 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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