GM05048
LCL from B-Lymphocyte
Description:
BETA GLOBIN REGION RESTRICTION POLYMORPHISMS
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Heritable Diseases |
| Class |
Mutations of the Hemoglobin Loci |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Race
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White
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Relation to Proband
|
proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis |
| |
| Remarks |
In blood: Hpa I x beta: 7.6/7.6 Kb; Bam HI x beta: 8.3/8.3 Kb; Hind III x gamma (G,A): 7.1, 2.7/7.8, 3.4 Kb; Hinc II x pseudo B1: 7.6/6.0 Kb |
| Rose S, Frye RE, Slattery J, Wynne R, Tippett M, Melnyk S, James SJ, Oxidative stress induces mitochondrial dysfunction in a subset of autistic lymphoblastoid cell lines Translational psychiatry4:e377 2013 |
| PubMed ID: 24690598 |
| |
| James SJ1, Rose S, Melnyk S, Jernigan S, Blossom S, Pavliv O, Gaylor DW., Cellular and mitochondrial glutathione redox imbalance in lymphoblastoid cells derived from children with autism. FASEB J23(8):2374-83 2009 |
| PubMed ID: 19307255 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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