GM03638
LCL from B-Lymphocyte
Description:
ACUTE LYMPHOCYTIC LEUKEMIA
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Heritable Diseases |
| Class |
Heritable Cancer Syndromes and other Cancers |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
B cell line 8392; same patient as GM03639C; positive for sIg and negative for T cell 65,000 MW glycoprotein; all cells show 8p- and 12p+; 2% are diploid, 90% tetraploid, and 8% are polyploid with random chromosome abnormalities |
| Lattier DL, States JC, Hutton JJ, Wiginton DA, Cell type-specific transcriptional regulation of the human adenosine deaminase gene. Nucleic Acids Res17:1061-76 1989 |
| PubMed ID: 2784203 |
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| Clarke BJ, Liao SK, Leeds C, Soamboonsrup P, Neame PB, Distribution of a hematopoietic-specific differentiation antigen of K562 cells in the human myeloid and lymphoid cell lineages. Cancer Res47:4254-9 1987 |
| PubMed ID: 3475169 |
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| Klobeck HG, Zachau HG, The human CK gene segment and the kappa deleting element are closely linked. Nucleic Acids Res14:4591-603 1986 |
| PubMed ID: 3086844 |
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| Mocikat R, Falkner FG, Mertz R, Zachau HG, Upstream regulatory sequences of immunoglobulin genes are recognized by nuclear proteins which also bind to other gene regions. Nucleic Acids Res14:8829-44 1986 |
| PubMed ID: 3786139 |
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| Verhoef V, Fridland A, Metabolic basis of arabinonucleoside selectivity for human leukemic T- and B-lymphoblasts. Cancer Res45:3646-50 1985 |
| PubMed ID: 2410098 |
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| Behrens UJ, Paronetto F, Elimination of the yeast Candida parapsilosis from lymphoid cells and monolayer cells in culture. In Vitro20:391-5 1984 |
| PubMed ID: 6586685 |
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| Royston I, Majda JA, Baird SM, Meserve BL, Griffiths JC, Human T cell antigens defined by monoclonal antibodies: the 65,000- dalton antigen of T cells (T65) is also found on chronic lymphocytic leukemia cells bearing surface immunoglobulin. J Immunol125:725-31 1980 |
| PubMed ID: 6993560 |
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| Huang CC, Hou Y, Woods LK, Moore GE, Minowada J, Cytogenetic study of human lymphoid T-cell lines derived from lymphocytic leukemia. J Natl Cancer Inst53:655-660 1974 |
| PubMed ID: 4547172 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
20% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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