GM01500
LCL from B-Lymphocyte
Description:
MYELOMA, MULTIPLE AMYLOIDOSIS, PRIMARY, INCLUDED; AL, INCLUDED
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Heritable Diseases |
| Class |
Other Disorders of Known Biochemistry |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| IMMUNOGLOBULIN PRODUCTION |
Lymphoid Culture Secretes IgG2 (Kappa) |
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| Remarks |
HLA type A2(A3),B12,Bw15; secretes IgG2 (Kappa); EBNA positive; negative for T cell 65,000 MW glycoprotein; positive for sIg and Ia (B cell) antigen; 78% 46,XY; 22% cells show random chromosome loss; 4% cells have a t(7;8); no reaction with OKT10 |
| Evans CE, Galasko CS, Ward C, Does myeloma secrete an osteoblast inhibiting factor? J Bone Joint Surg [Br]71:288-90 1989 |
| PubMed ID: 2925748 |
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| Tsujimoto Y, Overexpression of the human BCL-2 gene product results in growth enhancement of Epstein-Barr virus-immortalized B cells. Proc Natl Acad Sci U S A86:1958-62 1989 |
| PubMed ID: 2538824 |
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| Moore JS, Prystowsky MB, Hoover RG, Besa EC, Nowell PC, Defective T cell-mediated, isotype-specific immunoglobulin regulation in B cell chronic lymphocytic leukemia. Blood71:1012-20 1988 |
| PubMed ID: 2895674 |
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| Tong AW, Lee JC, Stone MJ, Characterization of a monoclonal antibody having selective reactivity with normal and neoplastic plasma cells. Blood69:238-45 1987 |
| PubMed ID: 3098320 |
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| Giallongo A, Feo S, Moore R, Croce CM, Showe LC, Molecular cloning and nucleotide sequence of a full-length cDNA for human alpha enolase. Proc Natl Acad Sci U S A83:6741-5 1986 |
| PubMed ID: 3529090 |
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| Mixter PF, Wu SV, Studnicka GM, Robinson RR, A simple, computer-assisted assay to detect isotype-specific regulation of human immunoglobulin synthesis. J Immunol Methods91:195-203 1986 |
| PubMed ID: 3755453 |
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| Nagarajan L, Louie E, Tsujimoto Y, ar-Rushdi A, Huebner K, Croce CM, Localization of the human pim oncogene (PIM) to a region of chromosome 6 involved in translocations in acute leukemias. Proc Natl Acad Sci U S A83:2556-60 1986 |
| PubMed ID: 3458216 |
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| Sun LK, Showe LC, Croce CM, Analysis of the 3' flanking region of the human c-myc gene in lymphomas with the t(8;22) and t(2;8) chromosomal translocations. Nucleic Acids Res14:4037-50 1986 |
| PubMed ID: 3714470 |
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| Goldstein M, Hoxie J, Zembryki D, Matthews D, Levinson AI, Phenotypic and functional analysis of B cell lines from patients with multiple myeloma. Blood66:444-6 1985 |
| PubMed ID: 3893568 |
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| Rauch J, Massicotte H, Tannenbaum H, Hybridoma anti-DNA autoantibodies from patients with rheumatoid arthritis and systemic lupus erythematosus demonstrate similar nucleic acid binding characteristics. J Immunol134:180-6 1985 |
| PubMed ID: 3964813 |
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| Showe LC, Ballantine M, Nishikura K, Erikson J, Kaji H, Croce CM, Cloning and sequencing of a c-myc oncogene in a Burkitt's lymphoma cell line that is translocated to a germ line alpha switch region. Mol Cell Biol5:501-9 1985 |
| PubMed ID: 3921823 |
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| Sutherland BM, Bennett PV, Freeman AG, Moore SP, Strickland PT, Transformation of human cells by DNAs ineffective in transformation of NIH 3T3 cells. Proc Natl Acad Sci U S A82:2399-403 1985 |
| PubMed ID: 3857589 |
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| Croce CM, Erikson J, ar-Rushdi A, Aden D, Nishikura K, Translocated c-myc oncogene of Burkitt lymphoma is transcribed in plasma cells and repressed in lymphoblastoid cells. Proc Natl Acad Sci U S A81:3170-4 1984 |
| PubMed ID: 6328505 |
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| Kaplan DR, Henkel TJ, Braciale V, Braciale TJ, Mycoplasma infection of cell cultures: thymidine incorporation of culture supernatants as a screening test. J Immunol132:9-11 1984 |
| PubMed ID: 6361127 |
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| Dwyer DS, Bradley RJ, Urquhart CK, Kearney JF, Naturally occurring anti-idiotypic antibodies in myasthenia gravis patients. Nature301:611-4 1983 |
| PubMed ID: 6402708 |
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| Shipp MA, Schwartz BD, Kannapell CC, Griffith RC, Scott MG, Ahmed P, Davie JM, Nahm MH, A unique DR-related B cell differentiation antigen. J Immunol131:2458-67 1983 |
| PubMed ID: 6415171 |
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| Hubbell, Cloning and analysis of double minute DNA from a human colon carcinoid cell line (from Gene Amplification, Cold Spring Ha rbor Lab, R.T. Schimke, ed). Gene Amplif1982 pp 193:2458-67 1982 |
| PubMed ID: 6415171 |
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| Karpas A, Fischer P, Swirsky D, Human myeloma cell line carrying a Philadelphia chromosome. Science216:997-9 1982 |
| PubMed ID: 7079750 |
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| Lane MA, Sainten A, Cooper GM, Stage-specific transforming genes of human and mouse B- and T- lymphocyte neoplasms. Cell28:873-80 1982 |
| PubMed ID: 6284377 |
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| Shoenfeld Y, Hsu-Lin SC, Gabriels JE, Silberstein LE, Furie BC, Furie B, Stollar BD, Schwartz RS, Production of autoantibodies by human-human hybridomas. J Clin Invest70:205-8 1982 |
| PubMed ID: 7085884 |
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| Yokochi T, Holly RD, Clark EA, B lymphoblast antigen (BB-1) expressed on Epstein-Barr virus-activated B cell blasts, B lymphoblastoid cell lines, and Burkitt's lymphomas. J Immunol128:823-7 1982 |
| PubMed ID: 6274961 |
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| Simmons JG, Fuller CR, Buchanan PD, Yount WJ, Distribution of surface, cytoplasmic and secreted IgG subclasses in human lymphoblastoid cell lines and normal peripheral blood lymphocytes. Scand J Immunol14:1-13 1981 |
| PubMed ID: 7313555 |
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| Croce CM, Linnenbach A, Hall W, Steplewski Z, Koprowski H, Production of human hybridomas secreting antibodies to measles virus. Nature288:488-9 1980 |
| PubMed ID: 6969366 |
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| Dolby TW, Devuono J, Croce CM, Cloning and partial nucleotide sequence of human immunoglobulin mu chain cDNA from B cells and mouse-human hybridomas. Proc Natl Acad Sci U S A77:6027-31 1980 |
| PubMed ID: 6777778 |
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| Croce CM, Shander M, Martinis J, Cicurel L, D'Ancona GG, Dolby TW, Koprowski H, Chromosomal location of the genes for human immunoglobulin heavy chains. Proc Natl Acad Sci U S A76:3416-9 1979 |
| PubMed ID: 114999 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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