GM01312
LCL from B-Lymphocyte
Description:
MYELOMA, MULTIPLE AMYLOIDOSIS, PRIMARY, INCLUDED; AL, INCLUDED
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Heritable Diseases |
| Class |
Other Disorders of Known Biochemistry |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Race
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Black/African American
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis |
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| Remarks |
Peripheral blood; HLA type A10,A11(A3), B12; same patient as GM01311A; derived from a patient with an IgA2 variety myeloma; secretes IgG in vitro; EBNA positive; no reaction with OKT10 |
| Sano H, Shiomi N, Imanishi K, Maie O, Shiomi T, DNA methylation in xeroderma pigmentosum. Mutat Res217:141-51 1989 |
| PubMed ID: 2918867 |
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| Tong AW, Lee JC, Stone MJ, Characterization of a monoclonal antibody having selective reactivity with normal and neoplastic plasma cells. Blood69:238-45 1987 |
| PubMed ID: 3098320 |
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| Goldstein M, Hoxie J, Zembryki D, Matthews D, Levinson AI, Phenotypic and functional analysis of B cell lines from patients with multiple myeloma. Blood66:444-6 1985 |
| PubMed ID: 3893568 |
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| Sutherland BM, Bennett PV, Freeman AG, Moore SP, Strickland PT, Transformation of human cells by DNAs ineffective in transformation of NIH 3T3 cells. Proc Natl Acad Sci U S A82:2399-403 1985 |
| PubMed ID: 3857589 |
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| Shipp MA, Schwartz BD, Kannapell CC, Griffith RC, Scott MG, Ahmed P, Davie JM, Nahm MH, A unique DR-related B cell differentiation antigen. J Immunol131:2458-67 1983 |
| PubMed ID: 6415171 |
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| Lane MA, Sainten A, Cooper GM, Stage-specific transforming genes of human and mouse B- and T- lymphocyte neoplasms. Cell28:873-80 1982 |
| PubMed ID: 6284377 |
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| Simmons JG, Fuller CR, Buchanan PD, Yount WJ, Distribution of surface, cytoplasmic and secreted IgG subclasses in human lymphoblastoid cell lines and normal peripheral blood lymphocytes. Scand J Immunol14:1-13 1981 |
| PubMed ID: 7313555 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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