GM00957
Amniotic fluid-derived cell line from Amniotic fluid
Description:
APPARENTLY HEALTHY AMNIOTIC FLUID CELL CULTURE
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Amniotic fluid
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Cell Type
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Amniotic fluid-derived cell line
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Transformant
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Untransformed
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Sample Source
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Amniotic fluid-derived cell line from Amniotic fluid
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis and Case history
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ISCN
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46,XX
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
6 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Remarks |
46,XX; 10% of cells show random chromosome loss |
| Chen YH, Grigelioniene G, Newton PT, Gullander J, Elfving M, Hammarsjö A, Batkovskyte D, Alsaif HS, Kurdi WIY, Abdulwahab F, Shanmugasundaram V, Devey L, Bacrot S, Brodszki J, Huber C, Hamel B, Gisselsson D, Papadogiannakis N, Jedrycha K, Gürtl-Lackner B, Chagin AS, Nishimura G, Aschenbrenner D, Alkuraya FS, Laurence A, Cormier-Daire V, Uhlig HH, Absence of GP130 cytokine receptor signaling causes extended Stüve-Wiedemann syndrome The Journal of experimental medicine217: 2019 |
| PubMed ID: 31914175 |
| |
| Wang H, Nakamura M, Abbott TR, Zhao D, Luo K, Yu C, Nguyen CM, Lo A, Daley TP, La Russa M, Liu Y, Qi LS, CRISPR-mediated live imaging of genome editing and transcription Science (New York, NY)217: 2019 |
| PubMed ID: 31488703 |
| |
| Postovit LM, Margaryan NV, Seftor EA, Kirschmann DA, Lipavsky A, Wheaton WW, Abbott DE, Seftor RE, Hendrix MJ, Human embryonic stem cell microenvironment suppresses the tumorigenic phenotype of aggressive cancer cells Proceedings of the National Academy of Sciences of the United States of America105:4329-34 2008 |
| PubMed ID: 18334633 |
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| Click EM, Balian G, Domain structure of human plasma and cellular fibronectin. Use of a monoclonal antibody and heparin affinity to identify three different subunit chains. Biochemistry24:6685-96 1985 |
| PubMed ID: 2417623 |
| |
| Vora S, Isozymes of human phosphofructokinase in blood cells and cultured cell lines: molecular and genetic evidence for a trigenic system. Blood57:724-32 1981 |
| PubMed ID: 6451249 |
| Passage Frozen |
6 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Amniotic Fluid Culture Medium |
| Serum |
none |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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