| mouse embryonic stem cell |
The frozen cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated mESC and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis. |