AG22719
Fibroblast from Skin, Foreskin
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Foreskin
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Foreskin
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Family Member
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1
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Family History
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N
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Relation to Proband
|
proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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|
| PDL at Senescence |
25 |
| PDL at Freeze |
2.49 |
| Passage Frozen |
1 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by LINE assay |
| |
| Remarks |
Foreskin fibroblast cell culture |
| Dardouri M, Aljnadi IM, Deuermeier J, Santos C, Costa F, Martin V, Fernandes MH, Gonçalves L, Bettencourt A, Gomes PS, Ribeiro IAC, Bonding antimicrobial rhamnolipids onto medical grade PDMS: A strategy to overcome multispecies vascular catheter-related infections Colloids and surfaces B, Biointerfaces217:112679 2022 |
| PubMed ID: 35816884 |
| |
| Dardouri M, Bettencourt A, Martin V, Carvalho FA, Colaço B, Gama A, Ramstedt M, Santos NC, Fernandes MH, Gomes PS, Ribeiro IAC, Assuring the Biofunctionalization of Silicone Covalently Bonded to Rhamnolipids: Antibiofilm Activity and Biocompatibility Pharmaceutics14:112679 2022 |
| PubMed ID: 36145584 |
| |
| Maïssa Dardouri, Ana Bettencourt, Victor Martin, Filomena A Carvalho, Catarina Santos, Nuno Monge, Nuno C Santos, Maria H Fernandes, Pedro S Gomes, Isabel A C Ribeiro, Using plasma-mediated covalent functionalization of rhamnolipids on polydimethylsiloxane towards the antimicrobial improvement of catheter surfaces Biomaterials Advances134:112679 2022 |
| PubMed ID: 35525746 |
| |
| Bettencourt AF, Tomé C, Oliveira T, Martin V, Santos C, Gonçalves L, Fernandes MH, Gomes PS, Ribeiro IAC, Exploring the potential of chitosan-based particles as delivery-carriers for promising antimicrobial glycolipid biosurfactants Carbohydrate polymers254:117433 2020 |
| PubMed ID: 33357906 |
| PDL at Senescence |
25 |
| Cumulative PDL at Freeze |
2.49 |
| Passage Frozen |
1 |
| Split Ratio |
1:20 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Ham's F12 Medium/Dulbecco Modified Eagles Medium, 1:1 mixture with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Supplement |
- |
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