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AG14730 LCL from B-Lymphocyte

Description:

APPARENTLY HEALTHY INDIVIDUAL

Affected:

No

Sex:

Male

Age:

8 YR (At Sampling)

  • Overview
  • Characterizations
  • Phenotypic Data
  • Publications
  • External Links
  • Culture Protocols

Overview

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Repository NIA Aging Cell Culture Repository
Subcollection "Intra-abdominal fat and risk of disease in children and adolescents" study
Apparently Healthy Collection
Biopsy Source Peripheral vein
Cell Type B-Lymphocyte
Tissue Type Blood
Transformant Epstein-Barr Virus
Sample Source LCL from B-Lymphocyte
Race White
Relation to Proband proband
Confirmation Clinical summary/Case history
ISCN 46,XY
Species Homo sapiens
Common Name Human
Remarks Participant in the "intra-abdominal fat and risk of disease in children and adolescents" study. The culture was initiated on 9/26/97 by transformation of lymphocytes with Epstein Barr virus. The culture grows in suspension and the cell morphology is spherical. The karyotype is 46,XY with 4% of the cells examined showing random chromosome loss. The legacy karyotype description shown in this Remark may not be representative of the current available product.

Characterizations

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IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis
 

Phenotypic Data

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Remarks Participant in the "intra-abdominal fat and risk of disease in children and adolescents" study. The culture was initiated on 9/26/97 by transformation of lymphocytes with Epstein Barr virus. The culture grows in suspension and the cell morphology is spherical. The karyotype is 46,XY with 4% of the cells examined showing random chromosome loss. The legacy karyotype description shown in this Remark may not be representative of the current available product.

Publications

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Cucco F, Sarogni P, Rossato S, Alpa M, Patimo A, Latorre A, Magnani C, Puisac B, Ramos FJ, Pié J, Musio A, Pathogenic variants in EP300 and ANKRD11 in patients with phenotypes overlapping Cornelia de Lange syndrome American journal of medical genetics Part A: 2019
PubMed ID: 32476269
 
Goran MI, Metabolic precursors and effects of obesity in children: a decade of progress, 1990-1999. Am J Clin Nutr73(2):158-71 2001
PubMed ID: 11157310
 
Goran MI, Bergman RN, Gower BA, Influence of total vs. visceral fat on insulin action and secretion in African American and white children. Obes Res9(8):423-31 2001
PubMed ID: 11500522
 
Goran MI, Gower BA, Longitudinal study on pubertal insulin resistance. Diabetes50(11):2444-50 2001
PubMed ID: 11679420
 
Herd SL, Gower BA, Dashti N, Goran MI, Body fat, fat distribution and serum lipids, lipoproteins and apolipoproteins in African-American and Caucasian-American prepubertal children. Int J Obes Relat Metab Disord25(2):198-204 2001
PubMed ID: 11410820
 
Huang TT, Johnson MS, Figueroa-Colon R, Dwyer JH, Goran MI, Growth of visceral fat, subcutaneous abdominal fat, and total body fat in children. Obes Res9(5):283-9 2001
PubMed ID: 11346669
 
Johnson MS, Figueroa-Colon R, Huang TT, Dwyer JH, Goran MI, Longitudinal changes in body fat in African American and Caucasian children: influence of fasting insulin and insulin sensitivity. J Clin Endocrinol Metab86(7):3182-7 2001
PubMed ID: 11443186
 
Li C, Johnson MS, Goran MI, Effects of low birth weight on insulin resistance syndrome in caucasian and African-American children. Diabetes Care24(12):2035-42 2001
PubMed ID: 11723079
 
Sun M, Gower BA, Bartolucci AA, Hunter GR, Figueroa-Colon R, Goran MI, A longitudinal study of resting energy expenditure relative to body composition during puberty in African American and white children. Am J Clin Nutr73(2):308-15 2001
PubMed ID: 11157329
 
Goran MI, Visceral fat in prepubertal children: Influence of obesity, anthropometry, ethnicity, gender, diet, and growth. Am J Human Biol11(2):201-207 1999
PubMed ID: 11533944

External Links

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dbSNP dbSNP ID: 19898

Culture Protocols

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Split Ratio 1:3
Temperature 37 C
Percent CO2 5%
Medium Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent
Serum 15% fetal bovine serum Not Inactivated
Substrate None specified
Subcultivation Method dilution - add fresh medium
Supplement -
Pricing
Commercial:
$257.00USD
Academic &
Non-profit:
$103.00USD
NIA Grantees:
$47.00USD
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How to Order
  • Ordering Instructions
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  • Statement of Research Intent Form
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