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AG13153 Fibroblast from Skin, Arm

Description:

GERONTOLOGY RESEARCH CENTER (GRC) CELL CULTURE COLLECTION
BALTIMORE LONGITUDINAL STUDY ON AGING (BLSA)

Affected:

No

Sex:

Male

Age:

30 YR (At Sampling)

  • Overview
  • Characterizations
  • Phenotypic Data
  • Publications
  • External Links
  • Culture Protocols

Overview

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Repository NIA Aging Cell Culture Repository
Subcollection GRC
Biopsy Source Arm
Cell Type Fibroblast
Tissue Type Skin
Transformant Untransformed
Sample Source Fibroblast from Skin, Arm
Race White
Family Member 2
Relation to Proband son
Confirmation Clinical summary/Case history
Species Homo sapiens
Common Name Human
Remarks The biopsy site is the mesial aspect of the mid-upper left arm, taken antemortem. The primary culture was initiated from explants of a 2 mm-punch biopsy. The cell morphology is fibroblast-like. A culture initiated from a biopsy taken 13 years earlier from this same donor is AG06234C.

Characterizations

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PDL at Freeze 4.18
Passage Frozen 5
 
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis
 

Phenotypic Data

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Remarks The biopsy site is the mesial aspect of the mid-upper left arm, taken antemortem. The primary culture was initiated from explants of a 2 mm-punch biopsy. The cell morphology is fibroblast-like. A culture initiated from a biopsy taken 13 years earlier from this same donor is AG06234C.

Publications

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Fleischer JG, Schulte R, Tsai HH, Tyagi S, Ibarra A, Shokhirev MN, Huang L, Hetzer MW, Navlakha S, Predicting age from the transcriptome of human dermal fibroblasts Genome Biology19: 2018
PubMed ID: 30567591
 
Kim Y1, Zheng X2, Ansari Z1, Bunnell MC1, Herdy JR1, Traxler L3, Lee H1, Paquola ACM4, Blithikioti C1, Ku M5, Schlachetzki JCM6, Winkler J7, Edenhofer F8, Glass CK9, Paucar AA1, Jaeger BN1, Pham S1, Boyer L1, Campbell BC1, Hunter T2, Mertens J10, Gage FH, Mitochondrial Aging Defects Emerge in Directly Reprogrammed Human Neurons due to Their Metabolic Profile Cell Reports23:2550-2558 2018
PubMed ID: 29847787
 
Arora G, Mezencev R, McDonald JF, Human cells display reduced apoptotic function relative to chimpanzee cells PloS one7:e46182 2012
PubMed ID: 23029431
 
Damodarasamy M, Vernon RB, Karres N, Chang CH, Bianchi-Frias D, Nelson PS, Reed MJ, Collagen extracts derived from young and aged mice demonstrate different structural properties and cellular effects in three-dimensional gels The journals of gerontology Series A, Biological sciences and medical sciences65:209-18 2010
PubMed ID: 20080876
 
Kriete A, Mayo KL, Yalamanchili N, Beggs W, Bender P, Kari C, Rodeck U, Cell autonomous expression of inflammatory genes in biologically aged fibroblasts associated with elevated NF-kappaB activity Immunity & ageing : I & A5:5 2008
PubMed ID: 18631391
 
Oh KS, Khan SG, Jaspers NG, Raams A, Ueda T, Lehmann A, Friedmann PS, Emmert S, Gratchev A, Lachlan K, Lucassan A, Baker CC, Kraemer KH, Phenotypic heterogeneity in the XPB DNA helicase gene (ERCC3): xeroderma pigmentosum without and with Cockayne syndrome HUMAN MUTATION27(11):1092-103 2006
PubMed ID: 16947863
 
Khan SG, Oh KS, Shahlavi T, Ueda T, Busch DB, Inui H, Emmert S, Imoto K, Muniz-Medina V, Baker CC, Digiovanna JJ, Schmidt D, Khadavi A, Metin A, Gozukara E, Slor H, Sarasin A, Kraemer KH, Reduced XPC DNA repair gene mRNA levels in clinically normal arents of xeroderma pigmentosum patients. Carcinogenesis27(1):84-94 2005
PubMed ID: 16081512
 
Reed MJ, Bradshaw AD, Shaw M, Sadoun E, Han N, Ferara N, Funk S, Puolakkainen P, Sage EH, Enhanced angiogenesis characteristic of SPARC-null mice disappears with age Journal of cellular physiology204:800-7 2005
PubMed ID: 15795937
 
Russanova VR, Hirai TH, Howard BH, Semirandom sampling to detect differentiation-related and age-related epigenome remodeling The journals of gerontology Series A, Biological sciences and medical sciences59:1221-33 2005
PubMed ID: 15699521
 
Russanova VR, Hirai TH, Tchernov AV, Howard BH, Mapping development-related and age-related chromatin remodeling by a high throughput ChIP-HPLC approach The journals of gerontology Series A, Biological sciences and medical sciences59:1234-43 2005
PubMed ID: 15699522
 
Karaman MW, Houck ML, Chemnick LG, Nagpal S, Chawannakul D, Sudano D, Pike BL, Ho VV, Ryder OA, Hacia JG, Comparative analysis of gene-expression patterns in human and African great ape cultured fibroblasts. Genome Res13(7):1619-1630 2003
PubMed ID: 12840040
 
Khan SG, Muniz-Medina V, Shahlavi T, Baker CC, Inui H, Ueda T, Emmert S, Schneider TD, Kraemer KH, The human XPC DNA repair gene: arrangement, splice site information content and influence of a single nucleotide polymorphism in a splice acceptor site on alternative splicing and function. Nucleic Acids Res30(16):3624-31 2002
PubMed ID: 12177305
 
Cristofalo VJ, Allen RG, Pignolo RJ, Martin BG, Beck JC, Relationship between donor age and the replicative lifespan of human cells in culture: a reevaluation. Proc Natl Acad Sci U S A95:10614-9 1998
PubMed ID: 9724752

External Links

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dbSNP dbSNP ID: 19186
GEO GEO Accession No: GSM244117
GEO Accession No: GSM244118

Culture Protocols

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Passage Frozen 5
Split Ratio 1:4
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent
Serum 15% fetal bovine serum Not inactivated
Supplement -
Pricing
Commercial:
$257.00USD
Academic &
Non-profit:
$103.00USD
NIA Grantees:
$47.00USD
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