AG13145
Fibroblast from Skin, Arm
Description:
GERONTOLOGY RESEARCH CENTER (GRC) CELL CULTURE COLLECTION
BALTIMORE LONGITUDINAL STUDY ON AGING (BLSA)
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
GRC |
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Biopsy Source
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Arm
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Arm
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Race
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White
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
4.36 |
| Passage Frozen |
4 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
The biopsy site is the mesial aspect of the mid-upper left arm, taken antemortem. The primary culture was initiated from explants of a 2 mm-punch biopsy. The cell morphology is fibroblast-like and the karyotype is 46,XY with 4% of the cells examined losing a Y chromosome. Cultures initiated from biopsies taken 15 and 6 years earlier from this same donor are AG05416 and AG11364, respectively. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Kamienieva I, Charzynska A, Duszynski J, Malinska D, Szczepanowska J, In search for mitochondrial biomarkers of Parkinson's disease: Findings in parkin-mutant human fibroblasts Biochimica et biophysica acta Molecular basis of disease1869:166787 2023 |
| PubMed ID: 37302428 |
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| Lindenkamp C, Plümers R, Osterhage MR, Vanakker OM, Van Wynsberghe J, Knabbe C, Hendig D, The Activation of JAK/STAT3 Signaling and the Complement System Modulate Inflammation in the Primary Human Dermal Fibroblasts of PXE Patients Biomedicines11:166787 2023 |
| PubMed ID: 37893046 |
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| Plümers R, Lindenkamp C, Osterhage MR, Knabbe C, Hendig D, Matrix Metalloproteinases Contribute to the Calcification Phenotype in Pseudoxanthoma Elasticum Biomolecules13:166787 2023 |
| PubMed ID: 37189419 |
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| Tiemann J, Lindenkamp C, Wagner T, Brodehl A, Plümers R, Faust-Hinse I, Knabbe C, Hendig D, The Consideration of Pseudoxanthoma Elasticum as a Progeria Syndrome Frontiers in bioscience (Landmark edition)28:55 2022 |
| PubMed ID: 37005749 |
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| Hung KF, Sidorova JM, Nghiem P, Kawasumi M, The 6-4 photoproduct is the trigger of UV-induced replication blockage and ATR activation Proceedings of the National Academy of Sciences of the United States of America28:55 2020 |
| PubMed ID: 32444488 |
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| Ly TD, Plümers R, Fischer B, Schmidt V, Hendig D, Kuhn J, Knabbe C, Faust I, Activin A-Mediated Regulation of XT-I in Human Skin Fibroblasts Biomolecules10:55 2020 |
| PubMed ID: 32295230 |
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| Tiemann J, Lindenkamp C, Plümers R, Faust I, Knabbe C, Hendig D, Statins as a Therapeutic Approach for the Treatment of Pseudoxanthoma Elasticum Patients: Evaluation of the Spectrum Efficacy of Atorvastatin In Vitro Cells10:55 2020 |
| PubMed ID: 33669724 |
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| Mamalis A, Koo E, Tepper C, Jagdeo J, MicroRNA Expression Analysis of Human Skin Fibroblasts Treated with High-Fluence LED Red Light Journal of biophotonics10:e201800207 2018 |
| PubMed ID: 30182520 |
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| Oh KS, Khan SG, Jaspers NG, Raams A, Ueda T, Lehmann A, Friedmann PS, Emmert S, Gratchev A, Lachlan K, Lucassan A, Baker CC, Kraemer KH, Phenotypic heterogeneity in the XPB DNA helicase gene (ERCC3): xeroderma pigmentosum without and with Cockayne syndrome HUMAN MUTATION27(11):1092-103 2006 |
| PubMed ID: 16947863 |
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| Khan SG, Oh KS, Shahlavi T, Ueda T, Busch DB, Inui H, Emmert S, Imoto K, Muniz-Medina V, Baker CC, Digiovanna JJ, Schmidt D, Khadavi A, Metin A, Gozukara E, Slor H, Sarasin A, Kraemer KH, Reduced XPC DNA repair gene mRNA levels in clinically normal arents of xeroderma pigmentosum patients. Carcinogenesis27(1):84-94 2005 |
| PubMed ID: 16081512 |
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| Khan SG, Metin A, Gozukara E, Inui H, Shahlavi T, Muniz-Medina V, Baker CC, Ueda T, Aiken JR, Schneider TD, Kraemer KH, Two essential splice lariat branchpoint sequences in one intron in a xeroderma pigmentosum DNA repair gene: mutations result in reduced XPC mRNA levels that correlate with cancer risk. Hum Mol Genet13(3):343-52 2003 |
| PubMed ID: 14662655 |
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| Khan SG, Muniz-Medina V, Shahlavi T, Baker CC, Inui H, Ueda T, Emmert S, Schneider TD, Kraemer KH, The human XPC DNA repair gene: arrangement, splice site information content and influence of a single nucleotide polymorphism in a splice acceptor site on alternative splicing and function. Nucleic Acids Res30(16):3624-31 2002 |
| PubMed ID: 12177305 |
| Cumulative PDL at Freeze |
9.36 |
| Passage Frozen |
4 |
| Split Ratio |
1:7 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Supplement |
- |
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